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Merck
모든 사진(1)

주요 문서

SRP0416

Sigma-Aldrich

T4 Beta-glucosyltransferase

recombinant, expressed in E. coli, ≥83% (SDS-PAGE)

동의어(들):

T4-phage beta-glucosyltransferase, UDP glucose-DNA ?-glucosyltransferase, hydroxymethyl cytosine

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크기 선택

100 μG
₩923,041

₩923,041


구입 가능 여부는 고객센터에 문의하십시오.


크기 선택

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100 μG
₩923,041

About This Item

UNSPSC 코드:
12352204
NACRES:
NA.32

₩923,041


구입 가능 여부는 고객센터에 문의하십시오.

생물학적 소스

Escherichia coli

재조합

expressed in E. coli

분석

≥83% (SDS-PAGE)

양식

aqueous solution

분자량

41.6 kDa

포장

pkg of 100 μg

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

bacteriophage T4 ... B-GT(1258765)

일반 설명

T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.

애플리케이션

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

생화학적/생리학적 작용

T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme.[1][2][3]

물리적 형태

Formulated in 200 mM imidazole and 20% glycerol.

픽토그램

Health hazardExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli.
S R KORNBERG et al.
The Journal of biological chemistry, 236, 1487-1493 (1961-05-01)
Chun-Xiao Song et al.
Nature biotechnology, 29(1), 68-72 (2010-12-15)
In contrast to 5-methylcytosine (5-mC), which has been studied extensively, little is known about 5-hydroxymethylcytosine (5-hmC), a recently identified epigenetic modification present in substantial amounts in certain mammalian cell types. Here we present a method for determining the genome-wide distribution
High resolution crystal structures of T4 phage beta-glucosyltransferase: induced fit and effect of substrate and metal binding.
Morera S
Journal of Molecular Biology, 311(3), 569-577 (2001)
Luis A Ortega-Ramirez et al.
Antibiotics (Basel, Switzerland), 9(3) (2020-03-04)
The resistance of Escherichia coli O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of Cymbopogon citratus (EO), have proven antibacterial activity
Bacteriophage T4 genome.
Miller ES
Microbiology and Molecular Biology Reviews, 67(1), 86-156 (2003)

문서

Explore tools for glycosyltransferase synthesis and modification of glycans, such as glycosyltransferases and nucleotide sugar donors.

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