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F1879

Sigma-Aldrich

Formaldehyde Dehydrogenase from Pseudomonas sp.

lyophilized powder, 1.0-6.0 units/mg solid

동의어(들):

FDH, Formaldehyde:NAD+ oxidoreductase

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모든 사진(3)

About This Item

CAS Number:
9028-84-6
효소 위원회 번호:
1.2.1.46 (BRENDA, IUBMB)
MDL number:
MFCD00131100
UNSPSC 코드:
12352204

형태

lyophilized powder

특이 활성도

1.0-6.0 units/mg solid

저장 온도

−20°C

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일반 설명

Formaldehyde Dehydrogenase (FDH) belongs to the medium-chain alcohol dehydrogenase family. It is encoded by the fdhA and fdhB gene in Pseudomonas aeruginosa. The FDH sequences of P. aeruginosa and P. putida are conserved and show homology. FDH exists as dimer and tetramer with zinc in their catalytic active site. The monomer corresponds to 350-400 amino-acid residues.

애플리케이션

Formaldehyde Dehydrogenase from Pseudomonas sp. has been used:

  • For standard curve generation for the demethylation-FDH assay of lysine specific demethylase 1 (LSD1) and in formaldehyde dehydrogenase (FDH) assay.
  • As a biosensor for the presence of formaldehyde in pharmaceuticals, waste water, vaccines and industrial products.
  • In coupled pectin methyl esterase (PME) enzyme assay.

생화학적/생리학적 작용

Formaldehyde dehydrogenase (FDH) is an enzyme that catalyzes the oxidation of formaldehyde to formate with NAD+ as an electron acceptor.Formaldehyde dehydrogenases are involved in the biological detoxification of formaldehyde, a toxic product in the metabolism of compounds like histidine, vanillate etc. They detoxify by converting formaldehyde to carbon-di-oxide. FDH plays a crucial role in regulating protein S-nitrosation in mammalian cells by decomposing S-nitrosoglutathione. They are also involved in the regulation of nitrosothiol homeostasis by catalyzing the reduction of endogenous nitrosylating agent S-nitrosoglutathione (GSNO).

단위 정의

One unit will oxidize 1.0 μmole of formaldehyde to formic acid per min at pH 7.5 at 37 °C.

물리적 형태

Lyophilized powder containing ~70% stabilizers as Mg2+, Ca2+, bovine serum albumin, glycine, and lysine

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

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문서 라이브러리 방문

The role of a formaldehyde dehydrogenase-glutathione pathway in protein S-nitrosation in mammalian cells
Haqqani A S, et al.
Nitric Oxide, 9(3), 172-181 (2003)
Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa
Zhang W, et al.
Protein Expression and Purification, 92(2), 208-213 (2013)
Structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa: the binary complex with the cofactor NAD+
Liao Y, et al.
Acta Crystallographica. Section F, Structural Biology Communications, 69(9), 967-972 (2013)
In Vitro Enzyme Assays for JmjC-Domain-Containing Lysine Histone Demethylases (JmjC-KDMs)
Tarhonskaya H, et al.
Current protocols in pharmacology, 80(1), 3-15 (2018)
Expression, purification, and characterization of formaldehyde dehydrogenase from Pseudomonas aeruginosa
Zhang W, et al.
Protein Expression and Purification, 92(2), 208-213 (2013)
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