E8656

Heat-Labile Enterotoxin, B subunit (LTB) from E. coli

recombinant, expressed in Pichia pastoris, >90% (SDS-PAGE), lyophilized powder

• 동의어(들): LTB
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• 재조합: expressed in Pichia pastoris
• Quality Level: 200
• 분석: >90% (SDS-PAGE)
• 형태: lyophilized powder
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

애플리케이션

Heat-Labile Enterotoxin, B subunit (LTB) from E. coli has been used in enzyme-linked immunosorbent assay (ELISA).

생화학적/생리학적 작용

Enterotoxigenic Escherichia coli causes diarrhea through its heat-labile enterotoxin (LT). The LT is a periplasmic protein composed of one A subunit (LTA, 27 kDa) and five non-covalently associated B subunits (LTB, 11.6 kDa each) forming a ring-like pentamer. LTB has high affinity towards the toxin receptor ganglioside G_M1, a glycosphingolipid found ubiquitously on the surface of mammalian cells. Ganglioside G_M1 facilitates the delivery of the A subunit to the cytosol of the target cell resulting in persistent synthesis of cAMP and subsequently diarrhea. This characteristic makes LTB a good label for microglial cells (due to the enrichment of ganglioside GM₁ on their cell surface). In addition, studies made mostly with animal models demonstrated that recombinant LTB could stimulate strong serum and mucosal immune responses against LT. Other studies have indicated that LTB could be used as a potent mucosal adjuvant.

특징 및 장점

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물리적 형태

Lyophilized from a solution containing Heat-Labile Enterotoxin, B subunit in 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN₃, and 1 mM sodium EDTA.

분석 메모

The activity is measured by ELISA using ganglioside G_M1-coated plates (Cat. No. G7641). LTB at various concentrations is incubated on the ganglioside G_M1 coated wells, followed by anti-cholera toxin antibody (Cat. No. C3062), and peroxidase-labeled goat anti-rabbit IgG (Cat.No. A0545) as the secondary antibody. Binding saturation of 50% may be achieved with = 0.12 μg/mL of LTB.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H312,H412
• 예방조치 성명서: P273 - P280 - P302 + P352 + P312 - P362 + P364 - P501
• Hazard Classifications: Acute Tox. 4 Dermal - Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter