추천 제품
사용
sufficient for 50 purifications
Quality Level
환경친화적 대안 제품 특성
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
환경친화적 대안 카테고리
일반 설명
Norgen Biotek′s Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and genomic DNA simultaneously from a single sample of plants. The total RNA and genomic DNA are both column purified in under 30 minutes using a single column. It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. Traditionally the RNA and DNA would be isolated from different aliquots of a sample, however this novel technology will allow for their simultaneous isolation from the same sample. This will not only save time, but will also be of a great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
애플리케이션
For simultaneous isolation of total RNA and DNA from the same plant sample.
Purified RNA and DNA are of the highest quality and can be used in a number of downstream applications including:
- Real-time PCR
- Southern blotting
- SNP analysis
- Sequencing
- AFLP
- RFLP
- RAPD
- Microsatellite analysis
- qRT-PCR
- Primer extension
- Expression array assays
- Next Generation Sequencing
특징 및 장점
- Total RNA and genomic DNA are column purified simultaneously in under 30 minutes.
- Reduces variability as RNA and DNA are isolated from a single plant sample.
- Isolate nucleic acids from small plant samples.
- Isolates total RNA including microRNAs.
- No phenol/chloroform extraction or alcohol precipitation.
- Isolate DNA-free plant RNA or RNA-free plant DNA
기타 정보
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
가장 최신 버전 중 하나를 선택하세요:
Ira Marton et al.
Plant physiology, 154(3), 1079-1087 (2010-09-30)
Zinc finger nucleases (ZFNs) are a powerful tool for genome editing in eukaryotic cells. ZFNs have been used for targeted mutagenesis in model and crop species. In animal and human cells, transient ZFN expression is often achieved by direct gene
프로토콜
The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
