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DUO92106

Sigma-Aldrich

Duolink® In Situ Orange Starter Kit Goat/Rabbit

동의어(들):

in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit

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크기 선택

1 KIT
₩2,403,419

₩2,403,419

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모든 사진(4)

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1 KIT
₩2,403,419

About This Item

UNSPSC 코드:
41116158
NACRES:
NA.32

₩2,403,419

구입 가능 여부는 고객센터에 문의하십시오.
벌크 견적 요청

제품 라인

Duolink®

Quality Level

100

기술

proximity ligation assay: suitable

형광

λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)

적합성

suitable for fluorescence

저장 온도

−20°C

관련 카테고리

애플리케이션

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.[1][2]

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Goat PLUS and Anti-Rabbit MINUS), orange detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

특징 및 장점

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

법적 정보

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS
  • DUO92005Duolink® In Situ PLA® Probe Anti-Rabbit MINUSSDS
  • DUO92003Duolink® In Situ PLA® Probe Anti-Goat PLUSSDS
  • DUO92007Duolink® In Situ Detection Reagents OrangeSDS
  • DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS
  • DUO82040Duolink® In Situ Mounting Medium with DAPISDS

픽토그램

Health hazardCorrosion
GHS08,GHS05

신호어

Danger

유해 및 위험 성명서

H290,H334,H412

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Resp. Sens. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Phosphorylation-mediated interactions with TOPBP1 couple
2 53BP1 and 9-1-1 to control the G1 DNA damage checkpoint
Pearl LH, et al.
bioRxiv, 510032-510032 (2019)
Thrombomodulin promotes focal adhesion kinase activation and contributes to angiogenesis by binding to fibronectin
Ysu YY, et al.
Oncotarget, 7(42), 68122-68139 (2016)
Peng Jiang et al.
PloS one, 10(6), e0128744-e0128744 (2015-06-09)
Exposure of platelets to collagen triggers the formation of a platelet clot. Pharmacological agents capable of inhibiting platelet activation by collagen are thus of potential therapeutic interest. Thrombus formation is initiated by the interaction of the GPIb-V-IX complex with collagen-bound
Xiaodong Xu et al.
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology, 49(11), 677-681 (2015-01-28)
To evaluate and compare the physical and chemical properties of four different dental cements under long-term water storage. A glass-ionomer cement (A:Fuji I), a resin reinforced glass-ionomer (B: Fuji Plus), a self-adhesive resin cement (C:G-Cem), and an etch & rinse
Gunisha Sagar et al.
Gut, 65(7), 1165-1174 (2015-06-11)
New-onset diabetes and concomitant weight loss occurring several months before the clinical presentation of pancreatic cancer (PC) appear to be paraneoplastic phenomena caused by tumour-secreted products. Our recent findings have shown exosomal adrenomedullin (AM) is important in development of diabetes
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문서

Duolink® PLA Technology for Neuroscience Applications

Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.

Duolink® PLA Troubleshooting Guide

Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

프로토콜

Duolink® PLA Fluorescence Protocol

This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

관련 콘텐츠

Duolink® PLA Applications

Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay

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