This kit is NOT suitable for flow cytometry. Please see our Duolink® flowPLA Mouse/Rabbit Starter Kits for flow cytometry: DUO94102 (Green), DUO94104 (Far Red), & DUO94105 (Violet).
추천 제품
제품 라인
Duolink®
Quality Level
기술
proximity ligation assay: suitable
형광
λex 594 nm; λem 624 nm (Texas Red®; Zeiss Filter set 31)
적합성
suitable for fluorescence
저장 온도
−20°C
애플리케이션
Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Red Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
The Duolink® In Situ Red Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
특징 및 장점
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
법적 정보
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies
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제품 번호
설명
SDS
- DUO92002Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)SDS
- DUO92004Duolink® In Situ PLA® Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H+L)SDS
- DUO92008Duolink® In Situ Detection Reagents RedSDS
- DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS
- DUO82040Duolink® In Situ Mounting Medium with DAPISDS
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
가장 최신 버전 중 하나를 선택하세요:
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문서
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
Things to consider for preparation, setup and execution of the Duolink® assay protocol
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
프로토콜
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
This page details the Duolink® In Situ Short Protocol for fluorescence detection
관련 콘텐츠
단백질 및 핵산 상호작용 시약과 단백질-RNA, 단백질-DNA, 단백질-단백질 상호작용 및 관련 애플리케이션 투자용 관련 자료
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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Hello, Is this kit also suitable for flow cytometry-based analysis of the data?
1 답변-
도움이 되었습니까?
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Can the Duolink kits be used with a Mouse IgM antibody?
1 답변-
Duolink kits may be compatible with a Mouse IgM antibody, provided that the antibody contains the light chain. The Anti-Mouse MINUS probe specifically interacts with mouse IgG (H+L) and the light chains associated with IgM. Therefore, if the Mouse IgM antibody includes the light chain, it should be suitable for use with the Duolink kits.
도움이 되었습니까?
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How many reactions can be done with this kit?
1 답변-
The Duolink™ starter kit contains all the components necessary to analyze up to 30 reactions in one single order. Please see the link below to review the full protocol:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/duolink-fluorescence-user-manual#DuolinkPLAFluorescenceProtocol도움이 되었습니까?
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I want to study the interaction of two cell surface proteins using PLA kit . My primaries are mouse and rabbit. Does the kit have the two secondary and also all the components that i would require to carry out the assay. Can you advice?
1 답변-
The Duolink® In Situ Red Starter Kit Mouse/Rabbit - DUO92101 contains the Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, affinity purified Donkey anti-Rabbit IgG, and Duolink® In Situ PLA® Probe Anti-Mouse MINUS, affinity purified Donkey anti-Mouse IgG (H+L) secondary antibodies and everything needed to conduct the PLA assay.
도움이 되었습니까?
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What is the expiry date of the kit contents?
1 답변-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.
For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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