35845
DCFHDA
BioReagent, suitable for fluorescence, ≥95% (HPLC)
동의어(들):
2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein
로그인조직 및 계약 가격 보기
모든 사진(1)
About This Item
실험식(Hill 표기법):
C24H14Cl2O7
CAS Number:
Molecular Weight:
485.27
Beilstein:
68488
MDL number:
UNSPSC 코드:
12352108
PubChem Substance ID:
NACRES:
NA.32
추천 제품
제품 라인
BioReagent
분석
≥95% (HPLC)
mp
232-234 °C (lit.)
solubility
DMSO: soluble
형광
λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
적합성
suitable for fluorescence
저장 온도
2-8°C
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
InChI key
VQVUBYASAICPFU-UHFFFAOYSA-N
일반 설명
2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.
애플리케이션
2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.
Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.
Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
dust mask type N95 (US), Eyeshields, Gloves
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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