You can use MTT to assess the intactness of isolated mitochondria. For this purpose, the CT01-5 Sigma-Aldrich MTT Reagent A is suitable. Additionally, the following publication may be of interest as a protocol reference: PLoS One. 2015 Jul 29;10(7):e0134139. This publication discusses the prevention of cisplatin-induced acute kidney injury and can provide valuable insights.
CS0390
Mitochondria Staining Kit
1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)
동의어(들):
JC-1 dye
크기 선택
₩1,053,164
크기 선택
About This Item
₩1,053,164
추천 제품
Quality Level
사용
kit sufficient for 200 tests (of 1 mL cell suspensions)
kit sufficient for 40 tests (of 5 mL cell suspensions)
포장
pkg of 1 kit
저장 조건
dry at room temperature
기술
flow cytometry: suitable
protein staining: suitable
형광
λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)
응용 분야
cell analysis
detection
검출 방법
fluorometric
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
애플리케이션
특징 및 장점
- A fast, simple, and convenient method for staining and assaying mitochondria intactness
- Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
- Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
- The shift in fluorescence is clearly detectable in both green and red channels
- Suitable for adherent cells as well as cells in suspension
- Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
- The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry
키트 구성품 전용
- DMSO 1 mL
- JC Staining Buffer 5X 120 mL
- JC-1 1 mg
- Valinomycin Ready Made .1 mL
Storage Class Code
10 - Combustible liquids
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
이미 열람한 고객
문서
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
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