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Merck
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주요 문서

CS0390

Sigma-Aldrich

Mitochondria Staining Kit

1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

동의어(들):

JC-1 dye

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1 KIT
₩1,053,164

₩1,053,164


구입 가능 여부는 고객센터에 문의하십시오.


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1 KIT
₩1,053,164

About This Item

UNSPSC 코드:
12352207
NACRES:
NA.32

₩1,053,164


구입 가능 여부는 고객센터에 문의하십시오.

Quality Level

사용

 kit sufficient for 200 tests (of 1 mL cell suspensions)
 kit sufficient for 40 tests (of 5 mL cell suspensions)

포장

pkg of 1 kit

저장 조건

dry at room temperature

기술

flow cytometry: suitable
protein staining: suitable

형광

λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)

응용 분야

cell analysis
detection

검출 방법

fluorometric

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.

애플리케이션

The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.

특징 및 장점

Kit offers:
  • A fast, simple, and convenient method for staining and assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
  • The shift in fluorescence is clearly detectable in both green and red channels
  • Suitable for adherent cells as well as cells in suspension
  • Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
  • The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry

키트 구성품 전용

제품 번호
설명

  • DMSO 1 mL

  • JC Staining Buffer 5X 120 mL

  • JC-1 1 mg

  • Valinomycin Ready Made .1 mL

Storage Class Code

10 - Combustible liquids


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

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Luteolin is a natural compound known for its anticancer effects on various human cancers by regulating signal transduction cascades. However, the effects of luteolin on human placental choriocarcinoma are not known. Results of the present study revealed that luteolin decreased
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A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm. Depending on the
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문서

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

질문

  1. Do you know which product is suitable for evaluating the intactness of isolated mitochondria?

    1 답변
    1. You can use MTT to assess the intactness of isolated mitochondria. For this purpose, the CT01-5 Sigma-Aldrich MTT Reagent A is suitable. Additionally, the following publication may be of interest as a protocol reference: PLoS One. 2015 Jul 29;10(7):e0134139. This publication discusses the prevention of cisplatin-induced acute kidney injury and can provide valuable insights.

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