CS0004

Nuclease micrococcal from Staphylococcus aureus

With reaction buffer and BSA solution

• 동의어(들): Nuclease micrococcal from Staphylococcus aureus, Endonuclease micrococcal, MNase, Micrococcal endonuclease
• CAS Number: 9013-53-0
• 효소 위원회 번호: 3.1.31.1 (BRENDA, IUBMB)
• MDL number: MFCD00131731
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: Staphylococcus aureus
• 양식: liquid
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Nuclease Micrococcal (MNase) is the extracellular nuclease of Staphylococcus aureus. The enzyme is an endo-exonuclease .This Endo-exonuclease preferentially digests single-stranded nucleic acids. Thus, nuclease micrococcal activity results in the production of mononucleotides and oligonucleotides with terminal 3′-phosphates. Nuclease Micrococcal is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes

애플리케이션

• Chromatin Immuno-Percipitation (ChIP) analysis
• Nucleosome preparation
• Removal of nucleic acids present in protein preparation
• Reduction of viscosity of cell lysates during non-mechanical cell lysis preparation
• RNA sequencing and transcriptomic profiling

생화학적/생리학적 작용

Hydrolyzes 5′-phosphodiester bonds of DNA.

기타 정보

Note: One μmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

안전성 정보

• Storage Class Code: 10 - Combustible liquids