JVM-2

96090515, human unknown/unspecified, Lymphoblastoid

• UNSPSC 코드: 41106514

속성

• 제품명: JVM-2, 96090515
• 생물학적 소스: human unknown/unspecified
• 성장 모드: Suspension
• 핵형: 46,XX,-8,+t(3;8)(p12-13;q12),t(11;14)(q13;q32)
• 형태학: Lymphoblastoid
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian B-prolymphocytic leukaemia, EBV-transformed

세포주 설명

JVM-2 has been derived from EBV and TPA treated mononuclear cells from a 63 year old female patient with B-prolymphocytic leukaemia. Thirty-three percent of cells were shown to be positive for surface lambda light chain, 37% for IgA, 29% for IgG and 3% for IgM and IgD. In addition, 50% were found to express cytoplasmic IgM and lambda light chain. Expression of the following leukocyte differentiation antigens was found: CD9, CD19, CD20, CD23, CD24, MA6, CD37, CD38, FMC1, FMC7, MHC Class I, FMC16, MHC Class II DR/DP/DQ. The immunoglobulin heavy-and light-chain genes showed the same pattern of rearrangement as in the original prolymphocytes. The cells are lymphoblastoid with large floating aggregates present. The cell line has been eradicated from mycoplasma at ECACC.

애플리케이션

Ig expression of neoplastic cells

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17

배양 배지

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Maintain cultures between 3-9 x 100,000/ml; 5% CO₂; 37°C.

기타 정보

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