EMT6/VRP/R

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse unknown/unspecified
• 포장: tube of 5 μg 96042319-DNA-5UG; pkg of vial of cells 96042319-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse mammary, drug-resistant

세포주 설명

The drug-resistant line EMT6/VRP/R was established from the parent cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in verapamil (see reference).

애플리케이션

Drug-resistance studies

배양 배지

MEM (EBSS) +2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 75 μg/ml verpamil + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37ºC. It is recommended to resuscitate cells in drug-free medium. Add drug after growth is established.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.