This cell line is prepared from cultures grown in RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week. Bringing cells out of freeze in a new medium is not recommended. A sudden change of medium may result in poor expansion or viability. The end user would have to determine suitability of another media formulation. In such situations, it is best to transition cells gradually to a new culture medium by adding increasing proportions of the new medium to the old with each split or subculture. See the link below to the ECACC product datasheet for additional information:
https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=93112520&collection=ecacc_gc#
추천 제품
제품명
A2780ADR Cell Line human, 93112520
생물학적 소스
human ovary
설명
Human Caucasian ovarian carcinoma
성장 모드
Adherent
핵형
Not specified
형태학
Epithelial
제품
Not specified
수용체
Not specified
기술
cell culture | mammalian: suitable
관련 질환(들)
cancer
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Human Caucasian ovarian carcinoma
세포주 설명
The adriamycin-resistant cell line A2780ADR has been developed by exposure of the parent A2780 cell line (Sigma catalogue no. 93112519) to adriamycin. A2780ADR is cross-resistant to melphalan and vinblastine. To retain resistance adriamycin has to be added to the media. The cells grow as a monolayer and in suspension in spinner cultures and are tumourigenic in immune deficient mice. Together with the cisplatin-resistant variant A2780cis these lines only differ in their exposure to a single drug and should facilitate the search for molecular changes responsible for the expression of pleiotropic drug resistance in human ovarian cancer.
애플리케이션
Study of drug transport, toxicity testing, drug resistance cancer studies
DNA 프로파일
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,14
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 15,16
CSF1PO: 10,11
D13S317: 13
D16S539: 11,14
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 15,16
배양 배지
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week.
계대배양 정규 작업
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established.
기타 정보
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
면책조항
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
가장 최신 버전 중 하나를 선택하세요:
-
Hi, I've just purchased the A2780ADR Cell Line human and had a few questions. Would there be any issues with using DMEM media instead of RPMI 1640 media? How is resistance measured in the cells likes and how do I insure that the resistance is retained?
1 답변-
도움이 되었습니까?
-
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
