CTLL-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93042610, mouse blood, Lymphoblast

• 동의어(들): CTLL-2 cell line
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: mouse blood
• 포장: tube of 5 μg 93042610-DNA-5UG; pkg of vial of cells 93042610-1VL
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Lymphoblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse C57b1/6 T cell

세포주 설명

CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.

애플리케이션

CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.[1][2]

배양 배지

RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 10⁵ cells/ml.

기타 정보

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