If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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추천 제품
생물학적 소스
human blood
포장
tube of 5 μg 93022307-DNA-5UG
pkg of vial of cells 93022307-1VL
성장 모드
Suspension
핵형
Carries homogeneous chromosomal abnormality (54,X)
형태학
Not specified
제품
Not specified
수용체
Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
기술
cell culture | mammalian: suitable
관련 질환(들)
cancer
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Human erythroleukaemia
세포주 설명
TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, but does not induce differentiation.
애플리케이션
TF1 cell line has been used-
- for tumorigenic studies
- to study the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) on proliferation and TNFR2 expression in TF1 cells[1]
- in a cell-based proliferation assay to study the biological activities of anti-IL-6R Nanobody ALX-0061[2]
- to study the induction of STAT5 phosphorylation by IL-3[3]
DNA 프로파일
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17
배양 배지
RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Foetal Bovine Serum (FBS). It is recommended to add fresh GM-CSF every 48 hours.
계대배양 정규 작업
Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.
기타 정보
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
가장 최신 버전 중 하나를 선택하세요:
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
1 답변-
도움이 되었습니까?
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How many Cell Line cells are in the vial?
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Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
도움이 되었습니까?
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Are the Cell Line cells tested for viral pathogens?
1 답변-
HPA Cultures does not perform any viral testing on the cell lines.
도움이 되었습니까?
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What medium should I use when I thaw the Cell Line cells?
1 답변-
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
도움이 되었습니까?
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How do I handle Cell Line frozen cells upon arrival?
1 답변-
Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
도움이 되었습니까?
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Are my Cell Line cells mycoplasma tested?
1 답변-
ECACC routinely tests all manufactured cell banks for mycoplasma.
도움이 되었습니까?
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What passage are my Cell Line cells?
1 답변-
If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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