46Br.1G1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92091814, human skin, Fibroblast

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human skin
• 포장: tube of 5 μg 92091814-DNA-5UG; pkg of vial of cells 92091814-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human skin fibroblast, SV40 T antigen transformed

세포주 설명

Derived from 46BR, a fibroblast from an individual with hypogammaglobulinaemia. The parent line was transformed with the plasmid pSV3gpt expressing SV40 T-antigen.

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; EDTA; 5% CO₂; 37°C.

기타 정보

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