CMT 93

89111413, mouse colon, Epithelial

• UNSPSC 코드: 41106514

속성

• 제품명: CMT 93, 89111413
• 생물학적 소스: mouse colon
• 성장 모드: Adherent
• 핵형: Hyperdiploid,modal no. 50
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse rectum carcinoma

세포주 설명

From a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage - source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1,000,000 cells inoculum) after 1 month.

애플리케이션

Tumourigenicity studies

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin; 5% CO₂; 37°C. Cells grow in clumps.

기타 정보

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면책조항

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.