IEC 18

88011801, rat intestine, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: IEC 18, 88011801
• 생물학적 소스: rat intestine
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Normal rat ileum

세포주 설명

Derived from normal epithelial cells of the rat ileum. Cells should undergo at least 20 population doublings. The line was established using the same techniques as for IEC6 (Sigma Catalogue number. 88071401). IEC 18 cells grow in small islands and do not reach confluency.

애플리케이션

Small bowel biology

배양 배지

DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

기타 정보

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