Detroit 532

87032602, human skin, Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: Detroit 532, 87032602
• 생물학적 소스: human skin
• 성장 모드: Adherent
• 핵형: 2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian, skin, Down syndrome

세포주 설명

Derived from the foreskin of a 2 month old Caucasian with Down Syndrome.

애플리케이션

Virus studies

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, up to 30 passages expected.

기타 정보

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