MM138 Cell Line human

10092321, human skin, Epithelial

• UNSPSC 코드: 41106514

속성

• 제품명: MM138 Cell Line human, 10092321, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• 생물학적 소스: human skin
• 포장: tube of 5 μg 10092321-DNA-5UG; pkg of vial of cells 10092321-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): metastasis
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human melanoma; from metastatic site - lymph node

세포주 설명

Metastatic Melanoma (metastatic site: lymph node). Cell Bank Australia reference number: CBA1345.

배양 배지

RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 10% fetal bovine serum (FBS).

계대배양 정규 작업

Optimal split ratio 1:4, MM138 has a relatively slow doubling time of around 60 hours. It does not become confluent and will detach after 6-7 days. If split too hard the doubling time is greatly increased. (Seeding density 0.8 x10⁴ cells/cm²). Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

기타 정보

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.