C8696

Cathepsin D from human liver

lyophilized powder, ≥250 units/mg protein (E1%/280)

• CAS Number: 9025-26-7
• 효소 위원회 번호: 3.4.23.5 (BRENDA, IUBMB)
• MDL number: MFCD00130749
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 형태: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥250 units/mg protein (E1%/280)
• 분자량: ~45 kDa
• 색상: white
• UniProt 수납 번호: P07339
• 저장 온도: −20°C
• 유전자 정보: human ... CTSD(1509)

설명

일반 설명

Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.

애플리케이션

Cathepsin D from human liver has been used:

• in β-secretase activity assay
• for enzymatic degradation of amyloid β 1-42
• in microinjection of human foreskin fibroblasts

생화학적/생리학적 작용

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

기타 정보

Contains α, β and γ isoenzymes as observed by isoelectric-focusing.

단위 정의

One unit will produce an increase in A₂₈₀ of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).

물리적 형태

Powder containing sodium phosphate buffer salt.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable