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Merck
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주요 문서

C-44010

Sigma-Aldrich

Lymphocyte Separation Medium 1077

500 ml

동의어(들):

Lymphocyte Medium, Separation Medium for Lymphocytes

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500 ML
₩212,135

₩212,135

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모든 사진(1)

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500 ML
₩212,135

About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

₩212,135

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벌크 견적 요청

양식

aqueous solution

포장

pkg of 500 ml

기술

cell culture | mammalian: suitable

배송 상태

ambient

저장 온도

room temp

일반 설명

Lymphocyte Separation Medium 1077

애플리케이션

Lymphocyte Separation Medium 1077 is intended for the separation of the portion of vital mononuclear cells from whole blood, buffy coats, bone marrow and several other starting materials, e.g. crude cell preparations, by means of low density gradient centrifugation. Lymphocyte separation Medium has a density of 1.077 g/ml at 20°C. It is sterile, ready-to use and based on Ficoll 400 and sodium diatrizoate providing optimal physiological parameters and low cytotoxicity.

품질

All lots of PromoCell Lymphocyte Separation Medium 1077 are subjected to comprehensive quality control tests. Each lot is routinely tested for function and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).

저장 및 안정성

Lymphocyte Separation Medium 1077 is light-sensitive! Store the medium at room temperature in the dark immediately after arrival. Do not freeze. If stored properly, the product is stable until the expiry date stated on the label.

계대배양 정규 작업

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픽토그램

Corrosion
GHS05

신호어

Warning

유해 및 위험 성명서

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

Lot/Batch Number

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Histopaque®-1119 sterile-filtered, density: 1.119

Sigma-Aldrich

11191

Histopaque®-1119

Fengyu Ming et al.
BioMed research international, 2020, 4658109-4658109 (2020-10-09)
Parkinson's disease (PD) is the second most common neurodegenerative disease in middle-aged and elderly people. However, the etiology and pathogenesis of PD are still unclear and there is a lack of reliable biomarkers for early molecular diagnosis. Parkin (encoded by

문서

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

질문

1–2 / 2 질문  

  1. What's the procedure of lymphocytes separation with this media and the quantity used .

    1 답변

    1. This is a product of PromoCell. Please see the instructions for use provided by the manufacturer:
      1. Fill Lymphocyte Separation Medium 1077 in a tube. Use 3 ml for a 15 ml tube and 20 ml for a 50 ml tube.
      2. Hold the tube at a 45° angle and carefully load the cell suspension to be separated on top of the separation medium.
      Note: Perform this step slowly but constantly. Take care that the two phases do not mix and a sharp border is present.
      For best results, use an electrical pipet aid with EX-function allowing the slow and constant efflux of the cell suspension from the pipet.
      3. Centrifuge the tube at 440 x g for 40 minutes at room temperature without brake.
      4. Carefully aspirate most of the supernatant without disturbing the layer of mononuclear cells in the interphase.
      5. Aspirate the ring of mononuclear cells from the interphase using a pipet. Keep the volume as small as possible.
      6. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 360 x g for 10 minutes.
      7. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 200 x g for 10 minutes X 2
      8. The mononuclear cell preparation is ready.

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  2. What percentage of lymphocytes/monocytes, etc. would be expected if they use it on fresh human blood?

    1 답변

    1. When utilizing the Lymphocyte Separation Medium 1077 (cat. #C-44010) on fresh human whole blood, the expected percentage of lymphocytes/monocytes typical for this sample is usually in the range of 70-90% of the enriched PBMCs.

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