B0631

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt

≥90%

• 동의어(들): 5-BrdUTP
• 실험식(Hill 표기법): C₉H₁₄BrN₂O₁₄P₃
• CAS Number: 102212-99-7
• Molecular Weight: 547.04
• MDL number: MFCD00057405
• UNSPSC 코드: 41106305
• PubChem Substance ID: 24891527
• NACRES: NA.51

속성

• 생물학적 소스: synthetic (organic)
• 분석: ≥90%
• 양식: powder
• solubility: water: 50 mg/mL, clear, colorless to faintly yellow
• 저장 온도: −20°C
• SMILES string: [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H14BrN2O14P3.Na.H/c10-4-2-12(9(15)11-8(4)14)7-1-5(13)6(24-7)3-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h2,5-7,13H,1,3H2,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
• InChI key: UWZGQOWZUSBZMC-UHFFFAOYSA-N

설명

일반 설명

5-Bromo-2′-deoxyuridine 5′-triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles.[1]

애플리케이션

5-Bromo-2′-deoxyuridine 5′-triphosphate (5-BrdUTP) may be used as a labeling TdT substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. BrdUTP is also used as an effective mutagenic agent. 5-BrdUTP labeling may be detected by immunological techniques or 5-BrdUTP may be derivitized by biotinylation or other methods for detection by fluorogenic or chromogenic methods.

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt has been used:

• in the labelling of 3′-OH termini of fragmented DNA with double stranded breaks in african green monkey kidney cells[2]
• to label cleaved DNA ends in apoptosis assay in avian tissue sections[3]
• for incorporation into mice prostates for immunohistochemistry studies[4]

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges