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추천 제품
사용
sufficient for 60 cell suspensions
포장
pkg of 1 kit
기술
flow cytometry: suitable
응용 분야
cell analysis
detection
검출 방법
fluorometric
배송 상태
wet ice
저장 온도
2-8°C
애플리케이션
The greater incorporation of Br-dUTP results in a stronger signal by flow cytometry when detected using a fluorescein-labeled anti-BrdU antibody.Propidium iodide/RNase A solution is included in the kit to counterstain the total DNA. APOBRDU has been used in flow cytometry assay in human non–small cell lung cancer.[1] APOBRDU has been used in cell cycle and apoptosis assay in HeLa cells,[2] and in tunel assay human neuroblastoma cell lines.[3]
특징 및 장점
Greater incorporation of Br-dUTP, resulting in improved detection than found by using biotin- or digoxigenin-conjugated dUTP or by using fluorochrome (fluorescein or BODIPY)-conjugated deoxynucleotides.
포장
The kit is shipped in two parts, both wet ice. Upon receipt, store APO-PART1 at −20 °C and APO-PART2 at 2-4 °C
원리
Br-dUTP is incorporated more readily into the DNA fragments than deoxyuridine triphosphate labeled with larger dyes such as FITC, biotin or digoxigenin. One of the biological characteristics that defines apoptosis is the degradation of genomic DNA into fragments of 180-200 bp, commonly called "DNA laddering". The fragmentation creates a large number of 3′-hydroxyl sites at the DNA breaks. This property is used in the APO-BRDU kit to identify apoptotic cells by labeling the 3′-hydroxyl sites with bromodeoxyuridine triphosphate (Br-dUTP). Br-dUTP is enzymatically attached to the 3-hydroxyl sites of double- or single-stranded DNA by terminal transferase (TdT). Non-apoptotic cells do not incorporate Br-dUTP due to the lack of available 3-hydroxyl sites.
키트 구성품 전용
제품 번호
설명
- Br-dUTP 480 μL
- Fluorescein PRB-1 antibody 300 μL
- Negative control cells 5 mL
- PI/RNase staining buffer 30 mL
- Positive control cells 5 mL
- Reaction buffer .6 mL
- Rinsing buffer 120 mL
- Terminal deoxynucleotidyl transferase (TdT) 45 μL
- Wash buffer 120 mL
모두 보기 (9)
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
3 - Flammable liquids
Flash Point (°F)
55.4 °F
Flash Point (°C)
13 °C
가장 최신 버전 중 하나를 선택하세요:
Cardenolide-induced lysosomal membrane permeabilization demonstrates therapeutic benefits in experimental human non-small cell lung cancers
Mijatovic T, et al.
Neoplasia, 8(5), 402-412 (2006)
The novel lipophilic camptothecin analogue gimatecan is very active in vitro in human neuroblastoma: a comparative study with SN38 and topotecan
Di Francesco AM, et al.
Biochemical Pharmacology, 70(8), 1125-1136 (2005)
Cdc7 is an active kinase in human cancer cells undergoing replication stress
Tenca P, et al.
The Journal of Biological Chemistry, 282(1), 208-215 (2007)
X Li et al.
Cell proliferation, 28(11), 571-579 (1995-11-01)
In situ presence of numerous DNA strand breaks is a typical feature of apoptotic cells. Selective DNA strand break induction by photolysis (SBIP) at sites that contain incorporated halogenated DNA precursors has recently been proposed as a method of analysing
Xiangjun Sun et al.
Experimental and therapeutic medicine, 17(3), 2334-2340 (2019-03-15)
The present study aimed to elucidate the underlying mechanism of neuroepithelial cell transforming 1 (NET-1), a member of the Ras homolog gene family, in hepatocellular carcinoma (HCC). To determine the association between the expression of NET-1 and the proliferation and
문서
암, 신경과학, 줄기 세포 연구 응용분야를 위한 세포 증식(BrdU, MTT, WST1), 세포 생존성, 세포 독성 실험용 세포 기반 분석법.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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