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Merck
모든 사진(3)

주요 문서

AC-6000

Sigma-Aldrich

HighPrep PCR Clean-up System

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About This Item

UNSPSC 코드:
41105500

양식

liquid

포장

pkg of 250 mL (AC-60250 (HighPrep PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep PCR (500 mL)))

저장 조건

do not freeze

저장 온도

2-8°C

일반 설명

Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.

The HighPrep PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.

애플리케이션

HighPrep PCR Clean-up System may be used in:
  • Post-PCR and post-enzymatic reaction clean-up[1][2][3][4][5]
  • NGS library preparation
  • Amplicon size selection for NGS[6]
  • Microarrays
  • PCR
  • Sanger sequencing
  • Restriction enzyme digestions, adapter ligations
  • Cloning

특징 및 장점

  • High recovery of >100 bp amplicons
  • Adaptable to high throughput liquid handling systems
  • Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
  • No centrifugation or filtration leads to higher and purer yields

저장 및 안정성

14 months when stored as directed

법적 정보

HighPrep is a trademark of MagBio Genomics, Inc.

면책조항

For Research Use Only. Not for Use in Diagnostic Procedures.

픽토그램

Health hazard

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Repr. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Shuba Varshini Alampalli et al.
PloS one, 12(12), e0188603-e0188603 (2017-12-09)
India has the third largest number of HIV-1-infected individuals accounting for approximately 2.1 million people, with a predominance of circulating subtype C strains and a low prevalence of subtype A and A1C and BC recombinant forms, identified over the past
The T-shirt microbiome is distinct between individuals and shaped by washing and fabric type
Sterndorff EB, et al.
Environmental Research, 109449-109449 (2020)
Lauren Bowen Reoma et al.
Annals of neurology, 86(6), 878-884 (2019-10-11)
JC virus (JCV) infection is a lytic infection of oligodendrocytes in progressive multifocal leukoencephalopathy; less common forms of central nervous system manifestations associated with JCV infection include granule cell neuronopathy, encephalopathy, and meningitis. Presented is the first case of fatal
Eva Baggesgaard Sterndorff et al.
Environmental research, 185, 109449-109449 (2020-04-12)
Activity of the microbial population in clothing causes unpleasant odor and textile deterioration. However, little is known about how the textile microbial community is shaped. In this study, we developed a method for extracting DNA from small amounts of detergent-washed
Soil bacterial diversity under conservation agriculture-based cereal systems in Indo-Gangetic Plains
Choudhary M, et al.
3 Biotech, 8, 304-304 (2018)

프로토콜

HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.

질문

후기

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활성 필터

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