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Merck
모든 사진(1)

주요 문서

A8438

Sigma-Aldrich

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

동의어(들):

Goat Anti-Rat IgG (whole molecule)–AP

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.46

생물학적 소스

goat

결합

alkaline phosphatase conjugate

항체 형태

affinity isolated antibody

항체 생산 유형

secondary antibodies

클론

polyclonal

양식

buffered aqueous glycerol solution

기술

direct ELISA: 1:30,000
immunohistochemistry (frozen sections): 1:50
western blot: 1:30,000

배송 상태

wet ice

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

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관련 카테고리

일반 설명

IgG, a monomer, is the predominant antibody circulating in blood which assist in phagocytic destruction of foreign microorganism. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 30 000) can be used in rat MPO (myeloperoxidase) ELISA. It may also use as secondary antibody in immunodetection method. Anti-rat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all rat immunoglobulins.

면역원

Purified rat IgG.

애플리케이션

Anti-rat IgG (whole molecule)–alkaline phosphatase antibody can be used in ELISA and western blot assays.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western blot was performed to analyze purified proteins over expressed in E.coli using alkaline-phosphatase-conjugated goat anti-rat IgG at a 1:1000 dilution. Western blot was developed using 5-bromo-4-chloro-3indolyl phosphate/nitro blue tetrazolium tablets (Sigma).

물리적 형태

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 10 mM glycine, 50% glycerol and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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문서 라이브러리 방문

Kailash C Pandey et al.
The Journal of biological chemistry, 279(5), 3484-3491 (2003-11-20)
The Plasmodium falciparum cysteine protease falcipain-2 is a trophozoite hemoglobinase and potential antimalarial drug target. Unlike other studied papain family proteases, falcipain-2 does not require its prodomain for folding to active enzyme. Rather, folding is mediated by an amino-terminal extension
Frank Lennartz et al.
Nature communications, 9(1), 3822-3822 (2018-09-22)
The quest to develop an effective malaria vaccine remains a major priority in the fight against global infectious disease. An approach with great potential is a transmission-blocking vaccine which induces antibodies that prevent establishment of a productive infection in mosquitos
Joya E Nahon et al.
Atherosclerosis, 281, 47-55 (2019-01-19)
Stabilin-1 (STAB1) is a scavenger receptor expressed on alternatively activated macrophages and sinusoidal endothelial cells. Its ligands include oxidized low-density lipoprotein (LDL) and the extracellular matrix glycoprotein SPARC and it is present in both human and murine atherosclerotic lesions. We
Chandra Ramakrishnan et al.
Scientific reports, 9(1), 1474-1474 (2019-02-08)
Almost any warm-blooded creature can be an intermediate host for Toxoplasma gondii. However, sexual reproduction of T. gondii occurs only in felids, wherein fertilisation of haploid macrogametes by haploid microgametes, results in diploid zygotes, around which a protective wall develops
Jianmin Zuo et al.
Journal of virology, 82(5), 2385-2393 (2007-12-21)
The DNase/alkaline exonuclease (AE) genes are well conserved in all herpesvirus families, but recent studies have shown that the AE proteins of gammaherpesviruses such as Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) exhibit an additional function which shuts down

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