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A4503

Sigma-Aldrich

Bovine Serum Albumin

Bovine Serum Albumin

cold ethanol fraction, pH 5.2, ≥96%

동의어(들):

Albumin bovine serum, BSA, Bovine albumin

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크기 선택

10 G
₩227,868
100 G
₩1,159,841
500 G
₩3,910,694
1 KG
₩5,711,710

₩227,868

예상 입고일2025년 5월 09일세부사항

모든 사진(5)

크기 선택

보기 변경
10 G
₩227,868
100 G
₩1,159,841
500 G
₩3,910,694
1 KG
₩5,711,710

About This Item

CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC 코드:
12352202
NACRES:
NA.27

₩227,868

예상 입고일2025년 5월 09일세부사항

생물학적 소스

bovine

분석

≥96%

양식

lyophilized powder

분자량

~66 kDa

정제법

cold ethanol fractionation

기원

USA origin

기술

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

불순물

BT and VSV virus, tested

손실

≤5%

pH

5.2

solubility

water: soluble (40 mg/ml)

UniProt 수납 번호

P02769

외래 활성

BT Virus, none detected
VSV Virus, none detected

저장 온도

2-8°C

유전자 정보

bovine ... ALB(280717)

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관련 카테고리

일반 설명

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids.[1][2] It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.[3]

애플리케이션

  • Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.[4]
  • It has been used in the cytotoxicity assay buffer.[5]
  • It has been used for the induction of protein overload nephropathy in rats.[6]

생화학적/생리학적 작용

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

제조 메모

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
0000439969
0000439969
0000403561
0000394757
0000394756

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COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Sensitization of tumor cells to NK cell-mediated killing by proteasome inhibition.
Hallett WH et al.
Journal of Immunology, 180, 163-163 (2008)
Cytochrome C effect on respiration of heart mitochondria: influence of various factors.
Toleikis A et al.
Bioscience Reports, 25, 387-387 (2005)
Isabella Marcomini et al.
Cell reports, 24(10), 2614-2628 (2018-09-06)
Multiple pathways regulate the repair of double-strand breaks (DSBs) to suppress potentially dangerous ectopic recombination. Both sequence and chromatin context are thought to influence pathway choice between non-homologous end-joining (NHEJ) and homology-driven recombination. To test the effect of repetitive sequences
Rapamycin worsens renal function and intratubular cast formation in protein overload nephropathy.
Coombes JD et al.
Kidney International, 68, 2599-2599 (2005)
Rui Zhang et al.
PLoS pathogens, 15(2), e1007329-e1007329 (2019-03-01)
Mycobacterial pathogens are the causative agents of chronic infectious diseases like tuberculosis and leprosy. Autophagy has recently emerged as an innate mechanism for defense against these intracellular pathogens. In vitro studies have shown that mycobacteria escaping from phagosomes into the
모든 관련된 서류 보기

문서

Albumin in Cell Culture

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

프로토콜

Nuclease S1 Enzymatic Assay

Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

모두 보기

관련 콘텐츠

Albumin from Bovine Serum Frequently Asked Questions (FAQs)

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

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질문

1–9 / 9 질문  

  1. Is this product biotin-free?

    1 답변

    1. This product is not tested for the presence of biotin. However, Albumin products prepared by cold ethanol fractionation are expected to be biotin-free.

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  2. Bonjour y a t-il un risque l'altération du produit si la bovin sérum albumine a été conservée pendant 15 heures en dehors du frigo dans une pièce a température ambiante ?

    1 답변

    1. Product A4503 (Bovine Serum Albumin) will not spoil following storage for 15 hours at ambient room temperature.

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  3. can A4503 product be used for primary neuron preparation?

    1 답변

    1. This product has not been evaluated for mammalian cell culture. Please see the datasheet for the recommended applications for this product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/384/276/a4503dat.pdf

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  4. Is Product No. A4503, Albumin from bovine serum, of US origin?

    1 답변

    1. It is typically from animals born and raised in the US. You may confirm this information by downloading the Certificate of Origin (CofO) for any lot of A4503 from our website. From the product detail page, simply type in the lot number in the box below the words Certificate of Origin, and click GO.

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  5. Is Product No. A4503, Albumin from bovine serum, tested and found to be endotoxin-free?

    1 답변

    1. No. If you need such a grade, we would recommend Product No. A8806.

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  6. Is Product No. A4053, Albumin from bovine serum, sterile? If not, how would recommend sterilizing it?

    1 답변

    1. This product is not necessarily sterile. Solutions of albumin cannot be autoclaved, so in order to sterilize it we would recommend that it be dissolved in the appropriate medium (water, saline or buffered saline), followed by sterile filtration through a low protein-binding membrane.

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  7. Is Product No. A4503, Albumin from bovine serum, tested and found to be protease-free?

    1 답변

    1. No. If you need such a grade, we would recommend Product No. A3059.

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  8. Do you offer a kit for conjugating ligands to bovine serum albumin (Product A4503)?

    1 답변

    1. Yes. It is our Product No. MBK1.

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  9. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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