The method of preparation is considered proprietary. However, as mentioned in this properties section of the Product Detail Page, this is Azurin from Pseudomonas aeruginosa. See the links below to review a sample Certificate of Analysis and the product data sheet, respectively:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A3672-1MG0000211689.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/306/100/a3672dat.pdf
추천 제품
생물학적 소스
Pseudomonas aeruginosa
Quality Level
양식
lyophilized powder
구성
Protein, ≥65% Lowry
농도
≥65.0% (Lowry)
기술
toxicology assay: suitable
solubility
water: soluble 1—1.1 mg/mL, clear, blue (light blue to blue)
UniProt 수납 번호
저장 온도
−20°C
유전자 정보
Pseudomonas aeruginosa ... AZU(878046)
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일반 설명
Research area: Apoptosis. Azurinis a periplasmic protein and is a homotetramer.[1]
애플리케이션
생화학적/생리학적 작용
Azurin acts as an electron donor for nitrite reductase in bacterial denitrification process.[1] It exhibits anticancer activity[1] as it hampers various independent signaling pathways associated with cancer progression. It binds to tumor suppressor protein p53 and induces cancer cell apoptosis or stalls cancer cell growth. Azurin disrupts angiogenesis by reducing the activity of VEGFR-2tyrosine kinase thereby inhibiting tumor growth. It has been observed to show cytotoxicity in human breast cancer cells and human melanoma cells.[5]
Azurin is a metalloprotein in the family of cupredoxins. It preferentially enters cancer cells over normal cells and induces apoptosis. Azurin has structural similarities to ephrinB2, and in fact binds the ephrin receptor tyrosine kinase EphB2 to initiate cell signaling that is involved in cancer progression. Azurin inhibits autophosphorlyation of the EphB2 tyrosine residue, interfering with upstream cell signaling and contributing to cancer cell growth inhibition.
물리적 형태
Lyophilized powder containing ammonium acetate buffer salts.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
Lot/Batch Number
Marcel Swart et al.
Chemistry & biodiversity, 9(9), 1728-1738 (2012-09-15)
UV/VIS Electron excitation spectra have been computed for large, realistic model systems of the blue copper protein family. Fully quantum-chemical calculations at the density-functional theory level employing polarized triple-ζ basis sets have been performed on systems of over 120 atoms
Interaction of p53 with Mdm2 and azurin as studied by atomic force spectroscopy
Funari G, et al.
Journal of Molecular Recognition, 23(4), 343-351 (2010)
Bacterial redox protein azurin induce apoptosis in human osteosarcoma U2OS cells
Yang DS, et al.
Pharmacological Research, 52(5), 413-421 (2005)
Krzysztof Kazimierczuk et al.
Journal of magnetic resonance (San Diego, Calif. : 1997), 223, 1-10 (2012-09-11)
The resolution of multidimensional NMR spectra can be severely limited when regular sampling based on the Nyquist-Shannon theorem is used. The theorem binds the sampling rate with a bandwidth of a sampled signal and thus implicitly creates a dependence between
Leandro Gammuto et al.
Microorganisms, 10(1) (2022-01-22)
Azurin is a bacterial-derived cupredoxin, which is mainly involved in electron transport reactions. Interest in azurin protein has risen in recent years due to its anticancer activity and its possible applications in anticancer therapies. Nevertheless, the attention of the scientific
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How do i know that this is azurin i mean any confirmatory test or anything else.
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