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Merck
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BCA1

Sigma-Aldrich

Bicinchoninic Acid Kit for Protein Determination

for 200-1000 μg/ml protein

동의어(들):

BCA protein assay

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크기 선택

1 KIT
₩708,166

₩708,166

재고 있음세부사항

모든 사진(1)

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1 KIT
₩708,166

About This Item

UNSPSC 코드:
12352106
NACRES:
NA.32

₩708,166

재고 있음세부사항

Quality Level

200

사용

sufficient for ~500 assays

저장 온도

2-8°C

애플리케이션

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples,[1] human embryonic kidney 293 cells[2] and luteal cells.[3]

특징 및 장점

  • Simple, sensitive colorimetric assay for proteins
  • Two stable reagents are used for the working solution
  • Faster and easier than the Lowry protein assay
  • Compatible with many detergents both ionic and nonionic
  • Less variation between different proteins than the Bradford dye-binding assay
  • Adaptable for use in microwell plates

원리

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

키트 구성품 전용

제품 번호
설명
  • Bicinchoninic Acid Solution 1 L
  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL
  • Protein Standard Solution 5 x 1

애플리케이션

제품 번호
설명
가격

픽토그램

CorrosionEnvironment
GHS05,GHS09

신호어

Danger

유해 및 위험 성명서

H290,H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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시험 성적서(COA)

Lot/Batch Number
0000412486
0000412486
0000389196
0000389196
0000368046

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

D Jantas et al.
Neurotoxicity research, 25(2), 208-225 (2013-10-10)
Tianeptine (Tian) possesses neuroprotective potential, however, little is known about the effect of this drug in models of neuronal apoptosis. In the present study, we aimed (1) to compare the neuroprotective capacities of some antidepressants (ADs) in the models of
TGFB1 modulates in vitro secretory activity and viability of equine luteal cells
Galvao A, et al.
Cytokine, 110, 316-327 (2018)
Salivary proteome patterns of individuals exposed to High Altitude
Jain S, et al.
Archives of Oral Biology, 96, 104-112 (2018)
Human OATP1B1 (SLCO1B1) transports sulfated bile acids and bile salts with particular efficiency
Toth B, et al.
Toxicology in vitro, 52, 189-194 (2018)
R E Brown et al.
Analytical biochemistry, 180(1), 136-139 (1989-07-01)
Protein quantitation based on bicinchoninic acid (BCA) is simple, sensitive, and tolerant to many detergents and substances known to interfere with the Lowry method. However, certain compounds often used during protein purification do interfere with the BCA protein assay. The
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문서

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

관련 콘텐츠

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

질문

1–3 / 3 질문  

  1. Can the kit be used for measuring the protein content of marine bacteria grown in artificial sea water?

    1 답변

    1. This product has not been tested for use with marine bacteria in artificial sea water. However, the publication below used the Bicinchoninic acid method to measure total protein in planktonic marine bacteria, indicating that this kit may be suitable.
      Determination of Total Protein Content of Bacterial Cells by SYPRO Staining and Flow Cytometry
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC91485/

      Note that there are a number of potential interfering substances that may impact results. Please see the link below to review the product datasheet, including a list of interfering substances beginning on page 4.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/246/793/bca1pis-ms.pdf

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  2. Does this kit rely on naked-eye colorimetric detection or does it require a fluorescent microscope?

    1 답변

    1. The kit is based on colorimetric, not fluorometric, detection. The color intensity should be measured at A562nm

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  3. Can a standard OD plate reader be used for the BCA1-1KT Kit, which offers a choice of assay tubes and 96-well plates as mentioned in the technical description? Additionally, would the Test tubes, 13 × 100 mm, Catalog Number CLS980013 not be needed?

    1 답변

    1. If a standard ELISA plate reader capable of measuring absorbance in the 560nm region is being used, the 96-well format should be suitable. The test tubes are recommended for larger volume analysis with cuvettes, but if the 96-well format is used, they will not be needed for the samples. However, they are also being utilized to make the BSA protein standard curve dilutions. If there is an alternative method for making the serial standard protein dilutions that ensures thorough mixing and utilizes a low-binding surface to prevent protein absorption, such as borosilicate glass and/or polypropylene tubes, that will be suitable as well.

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