A3429 and A3179 are the same solution but in different package sizes. No dilution is required for these products, but some customers may prefer a slightly weaker acid differentiation solution. The differentiation time may vary due to staining time and the thickness of the tissue sections being stained.
제품 A3429은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.
추천 제품
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.[1]
애플리케이션
Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
특징 및 장점
- Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
- Results are sharper and more rapid compared to other differentiation methods.[4]
- Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.
원리
In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.[1]
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
표적 기관
Eyes,Central nervous system
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point (°F)
71.6 °F
Flash Point (°C)
22 °C
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
Nuclear staining with alum hematoxylin
B D Llewellyn
Biotechnic & Histochemistry, 84(9), 159-177 (2009)
Pia M Teleman et al.
Acta obstetricia et gynecologica Scandinavica, 88(8), 927-932 (2009-07-07)
To outline possible associations between urinary incontinence (UI) and serum levels of steroid hormones in middle-aged women. Community-based observational study. All women aged 50-59 living in the Lund area by December 1995 were invited to a screening procedure. Sixty-four percent
Multispectral imaging as a tool for melanin detection
Kalleberg, et al.
Journal of Histotechnology, 38(1), 14-21 (2015)
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
Andres-Manzano, et al.
Methods in Molecular Biology, 1339 (2015)
M Jesús Andrés-Manzano et al.
Methods in molecular biology (Clifton, N.J.), 1339, 85-99 (2015-10-09)
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
