It is possible that either too much glacial acetic acid was added, or the acetic acid was added too quickly. When using the 0.5% Eosin Y solution sold as catalog number HT110216-500 ml, the instructions recommend adding 0.5 glacial acetic acid slowly to 100 ml of stain solution just prior to use. It's not uncommon to observe this in the lab. In most cases, the precipitate can be resolved by swirling the solution by hand or mixing it on a stir plate.
318906
Eosin Y solution
5 wt. % in H2O
동의어(들):
2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid red 87, Eosin yellowish solution
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모든 사진(2)
크기 선택
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500 ML
₩191,937
About This Item
실험식(Hill 표기법):
C20H6Br4Na2O5
CAS Number:
Molecular Weight:
691.85
Beilstein:
1443944
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47
추천 제품
양식
liquid
Quality Level
농도
5 wt. % in H2O
색상
red to very dark red
density
1.015 g/mL at 25 °C
λmax
518 nm
응용 분야
diagnostic assay manufacturing
hematology
histology
저장 온도
room temp
SMILES string
[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
InChI
1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
InChI key
SEACYXSIPDVVMV-UHFFFAOYSA-L
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Eosin Y, also known as Eosin yellowish is a widely used xanthene dye. It is a counterstain that is used to distinguish between various connective tissue fibers & matrices, and the cytoplasm of different types of cells. Eosin in combination with hematoxylin is useful to demonstrate the general histological architecture of a tissue.
애플리케이션
Eosin Y is suitable for use in hematoxylin and eosin (H&E) staining procedures for various tissue samples like lung nodule and kidney sections.
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Khalid Alyodawi et al.
Journal of cachexia, sarcopenia and muscle, 10(3), 662-686 (2019-03-28)
One of the principles underpinning our understanding of ageing is that DNA damage induces a stress response that shifts cellular resources from growth towards maintenance. A contrasting and seemingly irreconcilable view is that prompting growth of, for example, skeletal muscle
Peter A Raven et al.
Journal of cellular physiology, 234(11), 20634-20647 (2019-04-24)
The sonic hedgehog (SHH) signaling pathway plays an integral role in the maintenance and progression of bladder cancer (BCa) and SHH inhibition may be an efficacious strategy for BCa treatment. We assessed an in-house human BCa tissue microarray and found
Han Shih et al.
Macromolecular rapid communications, 34(3), 269-273 (2013-02-07)
The utility of visible-light-mediated polymerization in tissue engineering has been limited due to the necessary use of potentially cytotoxic coinitiator and comonomer. Here, we report a visible-light-mediated thiol-ene hydrogelation scheme using eosin-Y as the only photoinitiator. Under visible light exposure
Patricia S Nielsen et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 26(3), 404-413 (2012-11-24)
Cellular proliferation is correlated with the progression of melanoma. Accordingly, the proliferation index of H&E-stained thin melanomas was recently included in the staging system of the American Joint Committee on Cancer. Yet, the immunohistochemical markers of proliferation phosphohistone H3 and
Jie Du et al.
Fish & shellfish immunology, 34(1), 315-323 (2012-11-28)
Freshwater prawn Macrobrachium rosenbergii inoculated with 100 μl novel pathogen spiroplasma strain MR-1008 in logarithmic phase (10(8) spiroplasmas ml(-1)) were examined for alkaline phosphatase (AKP) activity, acid phosphatase (ACP) activity, superoxide dismutase (SOD) activity, catalase (CAT) activity, as well as
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a 1% Eosin Y solution in water using the product under the catalog number 318906 (Eosin Y solution 5wt% in water) was created and then diluted to 1% in water, the pH was lowered with acetic acid, but it immediately changed to a bright red color and produced a precipitate?
1 답변-
도움이 되었습니까?
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Is Product 318906 suitable for use in Hematoxylin and Eosin (H&E) staining procedures?
1 답변-
Product 318906 was not intended for use with H&E staining techniques. The dye content for the Eosin Y is 5% in water. For H&E staining, the concentration is typically 0.5 to 1.0 %. At 5 % dye content, the dye is much too concentrated for use with H&E staining. If used at such a high concentration, the cytoplasmic staining is likely to obscure the nuclear staining from the hematoxylin. A well stained H&E slide should show blue nuclear staining and typically at least 3 shades of Eosin staining. When used at 5% dye content, the cytoplasmic staining is likely to be a homogenous or monotone.The 5% Eosin solution has only one common application - determining sperm viability. In a typical semen analysis, 5% Eosin Y is used in conjunction with 10% Nigrosin. This combination of dyes is used to judge the viability of the sperm. A standard H&E procedure is then used to judge the morphology of the sperm. Sperm counts are typically performed without the use of a dye solution.It is possible to consider a 5% Eosin solution as a 10X staining solution. The product, however, is not suitability tested for use in either a sperm viability procedure or a H&E staining procedure.The following products have been suitability tested for use in H&E staining procedures and are considered suitable for use in In Vitro Diagnostic procedures. They are the preferred products for H&E staining. Most are formulated with 0.5% Eosin Y. The exception is Eosin Y with Phloxine B. For this formulation both the Eosin Y and the Phloxine B are formulated at 0.1% dye content.Eosin Y solution, alcoholicHT110116, HT110132, HT110180, HT1101128Eosin Y solution, aqueousHT110216, HT110232, HT110280, HT1102128Eosin Y solution, alcoholic, with phloxineHT110316, HT110332, HT110380, HT1103128
도움이 되었습니까?
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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