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Merck
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Key Documents

A0410

Sigma-Aldrich

Aminoallyl-dUTP sodium salt

≥85%, lyophilized powder

동의어(들):

5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP

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About This Item

실험식(Hill 표기법):
C12H20N3O14P3 · xNa+
CAS Number:
936327-10-5
Molecular Weight:
523.22 (free acid basis)
UNSPSC 코드:
41106305
NACRES:
NA.51

Quality Level

200

분석

≥85%

형태

lyophilized powder

배송 상태

dry ice

저장 온도

−70°C

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애플리케이션

Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
  • to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
  • for the generation of chromatin immunoprecipitation (ChIP) probes
  • in the reverse transcription of RNA for the synthesis of fluorescent labelled probes

생화학적/생리학적 작용

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is
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