추천 제품
생물학적 소스
goat
Quality Level
결합
peroxidase conjugate
항체 형태
affinity isolated antibody
항체 생산 유형
secondary antibodies
클론
polyclonal
종 반응성
human
기술
direct ELISA: 1:60,000
dot blot: 1:100,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
일반 설명
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. The gene encoding IgG gene cluster is mapped to human chromosome 14. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
The antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and is purified to remove unconjugated material.
Specificity and cross-reactivity of the Anti-Human IgG (Fc specific)-Peroxidase is determined by ELISA. The conjugate is specific for human IgG (Fc fragment) when tested against human IgA, IgG (Fab and Fc fragments), IgM, Bence Jones kappa, and lambda myeloma proteins. Furthermore, the conjugate shows no reactivity with mouse or rat IgG and yields reduced background with mouse or rat samples.
The antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and is purified to remove unconjugated material.
Specificity and cross-reactivity of the Anti-Human IgG (Fc specific)-Peroxidase is determined by ELISA. The conjugate is specific for human IgG (Fc fragment) when tested against human IgA, IgG (Fab and Fc fragments), IgM, Bence Jones kappa, and lambda myeloma proteins. Furthermore, the conjugate shows no reactivity with mouse or rat IgG and yields reduced background with mouse or rat samples.
특이성
Specificity of the Anti-Human IgG (Fc specific)- Peroxidase is determined by ELISA. The conjugate is specific for human IgG (Fc fragment) when tested against human IgA, IgG (Fab and Fc fragments), IgM, Bence Jones kappa, and lambda myeloma proteins. Cross-reactivity of the antibody-conjugate is determined by ELISA. The conjugate shows no reactivity with mouse or rat IgG and and yields reduced background with mouse or rat samples.
면역원
Fc segment of human IgG
애플리케이션
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (12 papers)
Western Blotting (3 papers)
Enzyme-linked immunosorbent assay (12 papers)
Western Blotting (3 papers)
Goat anti-human IgG conjugated to peroxidase is suitable for use in ELISA (1:60,000), Dot Blot (1:100,000), immunohistochemistry (1:150) and affinity chromatography applications.
Peroxidase-conjugated goat anti-human IgG (Fc′ specific) antibody was used to detect the level of IgG activity in human plasma samples after treatment with rifin proteins by ELISA (1:50000).
생화학적/생리학적 작용
Antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Glycosylation is critical for IgG function. IgG subclasses have therapeutic potential against bacterial and viral infections. Gene translocation of the immunoglobulin heavy chain is associated in pathogenesis of multiple myeloma.
물리적 형태
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
제조 메모
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
저장 및 안정성
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Molecular properties of human IgG subclasses and their implications for designing therapeutic monoclonal antibodies against infectious diseases.
Irani V, et al.
Molecular Immunology, 67(2)), 171-182 (2015)
Maurice Demanou et al.
BMC research notes, 3, 128-128 (2010-05-07)
Although arboviral infections including Chikungunya virus (CHIKV) are common in sub-Saharan Africa, data on their circulation and prevalence are poorly documented. In 2006, more than 400 cases of dengue-like fever were reported in Kumbo (Northwest Region of Cameroon). The aim
Mohamed S Abdel-Latif et al.
The American journal of tropical medicine and hygiene, 71(2), 179-186 (2004-08-13)
We used a pool of recombinant rifin proteins to pre-adsorb antibodies to rifin in the plasma of semi-immune African (Gabonese) adults and showed that this results in a reduction in the level of IgG antibody reactivity to variant surface antigens
Yvonne Rosenberg et al.
PloS one, 8(3), e58724-e58724 (2013-03-28)
Passive immunotherapy using anti-HIV broadly neutralizing monoclonal antibodies (mAbs) has shown promise as an HIV treatment, reducing mother-to-child-transmission (MTCT) of simian/human immunodeficiency virus (SHIV) in non-human primates and decreasing viral rebound in patients who ceased receiving anti-viral drugs. In addition
Tianlei Ying et al.
Nature communications, 6, 8223-8223 (2015-09-16)
The MERS-CoV is an emerging virus, which already infected more than 1,300 humans with high (∼36%) mortality. Here, we show that m336, an exceptionally potent human anti-MERS-CoV antibody, is almost germline with only one somatic mutation in the heavy chain.
문서
Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.
프로토콜
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
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