T8787

Triton^™ X-100

for molecular biology

• 동의어(들): t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• CAS Number: 9036-19-5
• Beilstein: 2315025
• MDL number: MFCD00128254
• UNSPSC 코드: 12161900
• PubChem Substance ID: 329826885
• NACRES: NA.21

속성

• 생물학적 소스: synthetic (organic)
• Grade: Molecular Biology; for molecular biology
• 설명: non-ionic
• 양식: liquid
• 분자량: micellar avg mol wt 80,000; average mol wt 625
• 응집 번호: 100-155
• 기술: protein purification: suitable; protein quantification: suitable; western blot: suitable
• 불순물: <1.00% water (Karl Fischer)
• pH: 9.7
• CMC: 0.2-0.9 mM (20-25°C)
• mp: 6 °C
• 전이 온도: cloud point 65 °C; pour point ~7 °C
• solubility: water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
• density: 1.06 g/mL at 25 °C (lit.)
• 양이온 미량물: Fe: <5 ppm; K: <0.05%; Na: <0.1%; heavy metals: <5 ppm
• HLB: 13.5
• 응용 분야: hematology; histology
• 외래 활성: DNase and RNase, none detected
• 저장 온도: room temp
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

설명

일반 설명

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[1][2]

애플리케이션

Triton^™ X-100 has been used:

• In immunohistochemistry for staining the Flat-mount retinas[3]
• Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay[4]
• To permeabilise cells during Immunofluorescent microscopic studies[5]
• As a positive control in LDH assay to determine the cell membrane integrity[6]
• For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure[7]
• For the preparation of outer membrane protein exctract[8]
• As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells[9]
• In the treatment of tissue sections for Immunofluorescence labeling[10]

생화학적/생리학적 작용

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

특징 및 장점

• Ideal for sensitive molecular biology research applications
• Tested for DNase and RNase
• Non-ionic nondenaturing detergent
• Superior wetting agent and emulsifier
• Highly versatile surfactant

기타 정보

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법적 정보

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

안전성 정보

• 픽토그램: GHS05,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H318,H410
• 예방조치 성명서: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): 483.8 °F - closed cup
• Flash Point (°C): 251 °C - closed cup
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter