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Merck
모든 사진(1)

주요 문서

P9187

Sigma-Aldrich

OPD

SIGMAFAST, peroxidase substrate, chromogenic, tablet

동의어(들):

OPD Peroxidase Substrate

로그인조직 및 계약 가격 보기

크기 선택

5 SETS
₩85,057
50 SETS
₩503,136

₩85,057


예상 입고일2025년 2월 17일세부사항

기존과 동일한 품질을 인하된 가격으로 - 어려운 예산 상황을 머크와 함께 극복하세요


크기 선택

보기 변경
5 SETS
₩85,057
50 SETS
₩503,136

About This Item

UNSPSC 코드:
12352204
NACRES:
NA.83

₩85,057


예상 입고일2025년 2월 17일세부사항

기존과 동일한 품질을 인하된 가격으로 - 어려운 예산 상황을 머크와 함께 극복하세요

제품명

SIGMAFAST OPD, tablet

양식

tablet

Quality Level

solubility

water: 0.5 tablet/10 mL, clear, colorless

저장 온도

2-8°C

애플리케이션

SIGMAFAST OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of peroxidase activity in enzyme immunoassays (EIA). OPD is the EIA substrate of choice as it exhibits high sensitivity. EIA applications utilizing OPD may be read in timed assays or stopped with dilute acid solutions for delayed readings.
SIGMAFAST OPD has been used in saliva- virus-like particles (VLP) binding assay.[1] It has also been used to develop enzyme linked immunosorbent assay (ELISA) plates for the detection of rituximab in rat serum samples.[2]

특징 및 장점

SIGMAFAST OPD Tablets require no additional buffers or steps to prepare an active substrate solution.

주의사항

This substrate is hygroscopic and light sensitive

재구성

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing OPD and urea hydrogen peroxide.
One OPD tablet and one urea hydrogen peroxide/buffer tablet, dissolved in 20 ml of water, provides 20 ml of ready-to-use substrate. When the tablet is dissolved in this manner, the final concentrations will be 0.4 mg/ml OPD, 0.4 mg/ml urea hydrogen peroxide, and 0.05 M phosphate-citrate, pH 5.0. Tablets are individually packaged in foil packets.

법적 정보

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Elisa Lobato et al.
Ecology and evolution, 7(11), 3645-3656 (2017-06-16)
Oceanic islands share several environmental characteristics that have been shown to drive convergent evolutionary changes in island organisms. One change that is often assumed but has seldom been examined is the evolution of weaker immune systems in island species. The
Courtney Woolsey et al.
Nature immunology, 22(1), 86-98 (2020-11-26)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for an unprecedented global pandemic of COVID-19. Animal models are urgently needed to study the pathogenesis of COVID-19 and to screen vaccines and treatments. We show that African green monkeys (AGMs)
Eliana Spilioti et al.
Environmental geochemistry and health, 39(4), 821-833 (2016-07-23)
Spa resorts are known for thousands of years for their healing properties and have been empirically used for the treatment of many inflammatory conditions. Mud is one of the most often used natural materials for preventive, healing and cosmetic reasons
Jeffrey D Whitman et al.
Nature biotechnology, 38(10), 1174-1183 (2020-08-29)
Appropriate use and interpretation of serological tests for assessments of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exposure, infection and potential immunity require accurate data on assay performance. We conducted a head-to-head evaluation of ten point-of-care-style lateral flow assays (LFAs)
Almahamoudou Mahamar et al.
Malaria journal, 16(1), 289-289 (2017-07-20)
Seasonal malaria chemoprevention (SMC) is a new strategy to reduce malaria burden in young children in Sahelian countries. It consists of the administration of full treatment courses of sulfadoxine-pyrimethamine plus amodiaquine to children at monthly intervals during the malaria season.

문서

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

프로토콜

간접 ELISA 및 포획 ELISA 실시에 관한 상세한 지침을 제공하는 ELISA 프로토콜 샌드위치 ELISA 및 간접 ELISA 실험을 실시하기 위한 권장 제품과 기법에 대해 자세히 알아봅니다.

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

질문

1–2 / 2 질문  
  1. What is an alternative for the SIGMAFAST OPD tablet set?

    What is an alternative for the SIGMAFAST OPD tablet set?

    1 답변
    1. Product P8287, o-Phenylenediamine dihydrochloride tablet will work, but it will require the addition of phosphate-citrate buffer and hydrogen peroxide. Suggested part numbers for these additional components are included in the datasheet: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/5/p8287dat.pdf

      도움이 되었습니까?

  2. Can I make the solution in large quantity and keep it in the fridge? Or should the solution be used right away because of its light sensitivity?

    1 답변
    1. The Substrate Solution is light-sensitive and should be protected from direct sunlight or UV sources in a
      tightly capped amber bottle. For best results, the Substrate Solution should be used within one hour.

      The Product Information Sheet is available for additional details:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/671/455/p9187pis-ms.pdf

      도움이 되었습니까?

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