62309

Lipase from Pseudomonas cepacia

powder, light beige, ≥30 U/mg

• 동의어(들): PCL, PS Lipase, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• CAS Number: 9001-62-1
• 효소 위원회 번호: 3.1.1.3 (BRENDA, IUBMB)
• EC Number: 232-619-9
• MDL number: MFCD00131509
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Pseudomonas cepacia)
• Quality Level: 100
• 양식: powder
• 특이 활성도: ≥30 U/mg
• 저장 조건: (Tightly closed. Dry)
• 기술: cell based assay: suitable
• 색상: light beige
• solubility: H₂O: 2 mg/mL, hazy, faintly yellow
• UniProt 수납 번호: A0A0J5WKE3
• 저장 온도: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

설명

일반 설명

Research area: cell-signaling

Lipase is a hydrolytic enzyme, found ubiquitously in nature. It belongs to the α/β-hydrolases fold family.[1] Lipase structure contains amphipathic helical lid domain in the active site that helps in interfacial activation of protein.[2]

애플리케이션

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from Pseudomonas cepacian has been used to:

• catalyze the degradation of polycaprolactone scaffold[3][4]
• catalyze the hydrolysis of Morita-Baylis-Hillman acetates during enzymatic kinetic resolution of racemic Morita-Baylis-Hillman adducts[5]
• as a standard for the generation of a calibration curve to determine the activity of lipase produced by microorganisms isolated from sludge derived from an urban wastewater treatment plant for ethanol production.[6]

생화학적/생리학적 작용

Lipases catalyze the hydrolysis of carboxylic ester bonds in triacylglycerols to yield glycerol and free fatty acids.[7][8] Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). Triacylglycerol lipases specifically hydrolyze the outer links of triacylglycerols and operate exclusively on the water-lipid interface.[9] Lipolytic products and intermediates formed during lipolysis are involved in various cell-signaling processes.[10] Lipases have broad substrate specificity and high enantioselectivity.[11] This property of lipase makes it a good catalyst in organic synthesis.[1] Lipases play a vital role in fat digestion and metabolism.[7]

단위 정의

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)

기타 정보

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Chemoenzymatic synthesis of (-)-carbocyclic 7-deazaoxetanocin G[12]

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)