62316

Lipase from Candida rugosa

powder, yellow-brown, ≥2 U/mg

• 동의어(들): CCL
• CAS Number: 9001-62-1
• 효소 위원회 번호: 3.1.1.3 (BRENDA, IUBMB)
• EC Number: 232-619-9
• MDL number: MFCD00131509
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: fungus (Candida rugosa)
• Quality Level: 100
• 형태: powder
• 특이 활성도: ≥2 U/mg
• 분자량: M_r ~67000
• 저장 조건: dry at room temperature
• 농도: ≤100%
• 기술: analytical sample preparation: suitable
• 색상: yellow-brown
• pH 범위: 6.5—7.5 (0.01 g/L)
• solubility: water: slightly soluble
• 응용 분야: sample preservation
• 저장 온도: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N
• 유전자 정보: fungus ... LAP1(2544)

설명

일반 설명

Research Area: Cell Signaling
The lipase enzyme is a naturally occurring enzyme present in both the stomach and pancreatic juice.[1] It is expressed and active in various tissues. For instance, hepatic lipases are found in the liver, hormone-sensitive lipases in adipocytes, lipoprotein lipase on the vascular endothelial surface, and pancreatic lipase in the small intestine. These lipases are classified within the alpha/beta-hydrolase fold superfamily of enzymes.[2]

애플리케이션

Lipase from Candida rugosa has been used to study and evaluate its effect on the anatomical structure and chemical composition of archaeological wood samples.[3] It has also been used to remove the oily dirt from a child′s tunic dated to the Coptic period and also to study the effect of the enzymatic treatment on the mechanical and optical parameters of linen using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), CIE-Lab values and ASTM method D5035.[4]

생화학적/생리학적 작용

Lipases, as a group of enzymes, are responsible for breaking down triglycerides into free fatty acids and glycerol, playing a pivotal role in the digestion, hydrolysis, and absorption of fat-soluble vitamins in pancreatic secretions.[2] It contributes to the maintenance of proper gallbladder function. These enzymes, derived from animals, plants, and various microorganisms, are known for their stability and are often described as nature′s catalysts. While microbial lipases are the primary choice for commercial applications, they possess the unique ability to hydrolyze fats into fatty acids and glycerols at the water-lipid interface and can also reverse this reaction in non-aqueous environments.[1] Elevated serum lipase levels can indicate pancreatitis. Understanding the role of lipase is essential for the pathophysiology of fat necrosis and acute and chronic pancreatitis. Lipases are also involved in the mechanism of some cholesterol-lowering medications.[2]

Candida rugosa lipase is known to catalyze hydrolysis reactions, especially the production of ricinoleic acid.

단위 정의

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)