추천 제품
제품명
1.1B4 human,
생물학적 소스
human pancreas (islets)
Quality Level
양식
liquid
성장 모드
Adherent
핵형
Modal chromosome number 67-71
형태학
Epithelial
제품
1.1B4 cells have been shown to express insulin, glucokinase and IAPP by immunocytochemistry. These cells express the GLUT1 glucose transporter.
수용체
Not specified
기술
cell culture | mammalian: suitable
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
A human pancreatic Beta cell: PANC-1 hybrid cell line. Insulin-secreting cell line.
세포주 설명
The hybrid cell line1.1B4 was formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line (ECACC catalogue number 87092802).
According to the literature (Vasu et al 2013) (Green et al 2015) 1.1B4 secretes a low level of insulin as detected by radioimmunoassay. 1.1B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology.
The STR profile of 1.1B4 is indistinguishable from the parental PANC-1 Cell Line indicating that the DNA from the pancreatic beta cell fusion partner is below the limit of detection of the STR profiling assay. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
According to the literature (Vasu et al 2013) (Green et al 2015) 1.1B4 secretes a low level of insulin as detected by radioimmunoassay. 1.1B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology.
The STR profile of 1.1B4 is indistinguishable from the parental PANC-1 Cell Line indicating that the DNA from the pancreatic beta cell fusion partner is below the limit of detection of the STR profiling assay. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
배양 배지
계대배양 정규 작업
Split sub-confluent cultures (70-80%) 1:2 to 1:6, i.e., seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approximately 20 hours. At confluence 105 cells/cm2 can be expected.
기타 정보
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
Srividya Vasu et al.
Islets, 5(4), 170-177 (2013-08-30)
The novel human-derived pancreatic β-cell line, 1.1B4 exhibits insulin secretion and β-cell enriched gene expression. Recent investigations of the cellular responses of this novel cell line to lipotoxicity and cytokine toxicity revealed similarities to primary human β cells. The current
Carina Levin et al.
International journal of molecular sciences, 22(18) (2021-09-29)
Beta thalassemia major (βT) is a hereditary anemia characterized by transfusion-dependency, lifelong requirement of chelation, and organ dysfunction. MicroRNA (miRNA) can be packed into extracellular vesicles (EVs) that carry them to target cells. We explored EV-miRNA in βT and their
Akiyo Yamauchi et al.
Journal of diabetes research, 2015, 173058-173058 (2015-03-15)
Reg (Regenerating gene) gene was originally isolated from rat regenerating islets and its encoding protein was revealed as an autocrine/paracrine growth factor for β cells. Rat Reg gene is activated in inflammatory conditions for β cell regeneration. In human, although
Kate L White et al.
Science advances, 6(50) (2020-12-11)
Characterizing relationships between cell structures and functions requires mesoscale mapping of intact cells showing subcellular rearrangements following stimulation; however, current approaches are limited in this regard. Here, we report a unique application of soft x-ray tomography to generate three-dimensional reconstructions
Patchara Rattanaporn et al.
PeerJ, 8, e9298-e9298 (2020-06-27)
Diabetes mellitus (DM) is a common complication found in β-thalassemia patients. The mechanism of DM in β-thalassemia patients is still unclear, but it could be from an iron overload and increase of some cytokines, such as interleukin1-β (IL-1β) and tumor
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