E7750

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

commercial grade, powder

• 동의어(들): N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC, EDC hydrochloride, WSC hydrochloride
• 실험식(Hill 표기법): C₈H₁₇N₃ · HCl
• CAS Number: 25952-53-8
• Molecular Weight: 191.70
• Beilstein: 5764110
• EC Number: 247-361-2
• MDL number: MFCD00012503
• UNSPSC 코드: 12352111
• PubChem Substance ID: 24894654
• NACRES: NA.31

속성

• Quality Level: 300
• Grade: commercial grade
• 형태: powder
• 기술: Northern blotting: suitable; bioconjugation: suitable
• 색상: white to off-white
• mp: 110-115 °C (lit.)
• solubility: H₂O: ≤100 mg/mL
• 저장 온도: −20°C
• SMILES string: Cl.CCN=C=NCCCN(C)C
• InChI: 1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
• InChI key: FPQQSJJWHUJYPU-UHFFFAOYSA-N

설명

일반 설명

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC HCl), commonly referred to as EDAC, is a highly potent and extensively utilized water-soluble reagent in the realm of chemical and biochemical research, playing a crucial role in facilitating the formation of amide bonds. Within the domain of peptide synthesis, EDC HCl demonstrates notable efficiency by catalyzing the coupling of amino acids through their carboxyl and amine groups, thereby facilitating the creation of peptide backbones with specific sequences and functionalities. This particular attribute is highly valuable in the production of peptides for various research purposes. Expanding beyond peptides, EDC HCl′s influence extends to the construction of immunogens, where it actively participates in the covalent attachment of haptens (small molecules that elicit an immune response) to carrier proteins. This involvement proves instrumental in the research and development of vaccines.

The versatility of EDC HCl further manifests in its capacity to modify nucleic acids, allowing for the labeling of DNA and RNA through their 5′ phosphate groups. This functionality enhances the visualization, tracking, and analysis of these crucial molecules, contributing significantly to the progression of nucleic acid research. Moreover, EDC HCl serves as a vital biomolecule bridge, acting as a crosslinker that connects amine-reactive NHS-esters of biomolecules to carboxyl groups. This technique is particularly valuable in protein conjugation, enabling the creation of hybrid molecules with novel properties and functions. The underlying mechanism of EDC HCl involves its reaction with a carboxyl group, forming an unstable intermediate that actively seeks an amine partner. The delicate balance of this reaction emphasizes the need for optimizing conditions to ensure efficient conjugation. The assistance of N-hydroxysuccinimide (NHS) further enhances EDC HCl′s capabilities by stabilizing the intermediate and enabling two-step conjugation procedures, offering greater flexibility and control, especially when dealing with complex biomolecules.

애플리케이션

• N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used for the formation of FND (fluorescent nanodiamonds)-transferrin bioconjugates.
• It has been used for crosslinking polyethylenimine to gold particles.
• It has been used as a carbodiimide linkage agent for coating of carboxylated polystyrene beads with biotinylated BSA (bovine serum albumin).

생화학적/생리학적 작용

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

특징 및 장점

Versatile and adaptable for a wide variety of laboratory and research applications

기타 정보

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안전성 정보

• 픽토그램: GHS06,GHS08,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H311,H315,H317,H373,H410
• 예방조치 성명서: P260 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
• 표적 기관: Stomach,large intestine,lymph node
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves