A7431

Agarose

BioReagent, for molecular biology, Wide range/Standard 3:1

• 동의어(들): 3,6-Anhydro-α-L-galacto-β-D-galactan
• CAS Number: 9012-36-6
• EC Number: 232-731-8
• MDL number: MFCD00081294
• UNSPSC 코드: 41105317
• PubChem Substance ID: 24891233
• NACRES: NA.25

속성

• 생물학적 소스: algae (marine)
• Quality Level: 200
• Grade: for molecular biology
• 제품 라인: BioReagent
• 형태: powder
• 기술: electrophoresis: suitable
• EEO: ≤0.13
• mp: ≤90 °C
• 전이 온도: gel point 32.5-38 °C (4% gel)
• 겔 강도: ≥1200 g/cm² (4% gel)
• 음이온 미량물: sulfate (SO₄^2-): ≤0.15%
• 외래 활성: DNase, RNase, NICKase, none detected
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

설명

애플리케이션

Specially formulated to form strong, flexible gels for separation of small (≤1 kb) PCR products, DNA and RNA. Stronger gels make gel-handling easier. Sharp resolution is produced with DNA fragments ranging from 10-1500 bp.

분석 메모

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)