추천 제품
Grade
ACS reagent
Quality Level
vapor density
1.55 (vs air)
vapor pressure
0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)
분석
≥99.5%
형태
liquid
autoignition temp.
932 °F
기술
PCR: suitable
색상
APHA: ≤10
refractive index
n20/D 1.447 (lit.)
pH
4-10 (20 °C, 200 g/L)
bp
210 °C (lit.)
mp
2-3 °C (lit.)
전이 온도
freezing point 2.0-3.0 °C
density
1.134 g/mL at 25 °C (lit.)
SMILES string
NC=O
InChI
1S/CH3NO/c2-1-3/h1H,(H2,2,3)
InChI key
ZHNUHDYFZUAESO-UHFFFAOYSA-N
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애플리케이션
The addition of formamide may enhance the specificity and efficiency of amplification of PCR (polymerase chain reaction). It may be used as an ammonia equivalent for the palladium-catalyzed aminocarbonylation of 1-bromo-3-trifluoromethylbenzene to form 3-(trifluoromethyl)benzamide.
Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Carc. 2 - Repr. 1B - STOT RE 2 Oral
표적 기관
Blood
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Flash Point (°F)
305.6 °F
Flash Point (°C)
152 °C
개인 보호 장비
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Formamide can dramatically improve the specificity of PCR.
Nucleic Acids Research, 18(24), 7465-7465 (1990)
Science of Synthesis: Houben-Weyl Methods of Molecular Transformations, 21, 126-126 (2014)
Microwave spectrum and molecular structure of formamide.
J. Chem. Phys. , 32(1), 158-165 (1960)
Nucleic acids research, 4(5), 1539-1552 (1977-01-01)
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is
Solubilization in formamide protects RNA from degradation.
Nucleic Acids Research, 20(14), 3791-3791 (1992)
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