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Merck
모든 사진(1)

주요 문서

11966006001

Roche

TUNEL Dilution Buffer

pH 7.2-7.4 (20 °C), suitable for detection, pkg of 2 × 10 mL

동의어(들):

TUNEL, transferase dUTP nick end labeling

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About This Item

UNSPSC 코드:
41116100

포장

pkg of 2 × 10 mL

제조업체/상표

Roche

pH

7.2-7.4 (20 °C)

응용 분야

detection

저장 온도

2-8°C

일반 설명

The TUNEL dilution buffer can be used in combination with the in-situ cell death detection kits. It can be used to dilute terminal deoxynucleotidyl transferase (TdT) for the TUNEL reaction in special applications and also to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.

애플리케이션

TUNEL Dilution Buffer has been used:
  • to dilute terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) enzyme (TdT) in preparing TUNEL reaction mixture for TUNEL critical commercial assays[1]
  • to quantify apoptosis in rat kidney[2]
  • to dilute the terminal deoxynucleotidyl transferase enzyme solution to identify DNA fragmentation in oligodendrocytes in vitro[3]
  • for TUNEL assays[4]

TUNEL dilution buffer has been used in terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays.[5]

제조 메모

Working concentration: Working concentration of conjugate depends on application and substrate.


The following concentrations should be taken as a guideline:
  • ELISA: 100 mU/ml
  • Western blot: 100mU/ml


Storage conditions (working solution): Dilutions of TdT should be prepared immediately before use and should not be stored.
Keep TUNEL reaction mixture on ice until use.

기타 정보

For life science research only. Not for use in diagnostic procedures.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B Inhalation

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


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