DTTP-RO

dTTP

PCR Grade, sodium salt

• 동의어(들): Thymidine 5′-triphosphate sodium salt solution, TTP
• CAS Number: 18423-43-3
• MDL number: MFCD00080338
• UNSPSC 코드: 12352302
• PubChem Substance ID: 329798929

속성

• Quality Level: 100
• 분석: 99% (dNTP)
• 형태: solution
• 사용: sufficient for 125,000 standard reactions (03732711001); sufficient for 31,250 standard reactions (11969048001); sufficient for 6,250 standard reactions (11934546001)
• 포장: pkg of 1,250 μL (11969048001 [100 mM]); pkg of 4 × 1,250 μL (03732711001 [4x100 mM]); pkg of 250 μL (11934546001 [100 mM])
• 제조업체/상표: Roche
• 농도: <0.9% (dNDP); 100 mM
• 저장 온도: −20°C
• SMILES string: [Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
• InChI: 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
• InChI key: KAELIXTVSANDSE-UHFFFAOYSA-N

설명

일반 설명

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.

dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

애플리케이션

dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

품질

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

물리적 형태

solution of dTTP in water (pH 8.3).

제조 메모

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

기타 정보

For general laboratory use.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash