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Merck
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주요 문서

SCT210

Sigma-Aldrich

BioTracker FerroOrange Live Cell Dye

Live Cell imaging probe that specifically detects labile ferrous (Fe2+) ions in live cells.

동의어(들):

senescent cells IHC kit

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모든 사진(1)

About This Item

UNSPSC 코드:
12352207
NACRES:
NA.21
제품 SCT210은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.

양식

lyophilized

응용 분야

cell analysis

검출 방법

fluorometric

관련 카테고리

일반 설명

Iron, the most abundant transition metal in our bodies, is involved in a number of biologically important processes such as respiration, oxygen transport, and energy production in collaboration with oxygen. In living cells, iron exists mainly as ferrous (Fe2+) and ferric (Fe3+) ionic forms. As a major contributor to oxidative damage of cells, Fe2+ is implicated in serious diseases such as cancers and neurodegenerative disorders, because of its ability to produce harmful reactive oxygen species via contact with oxygen, superoxide, and hydrogen peroxide (H2O2).BioTracker FerroOrange Live Cell Dye is an orange fluorescent probe that specifically detects labile iron (II) ions (Fe2+) only. The intensity of fluorescence does not increase in the presence of iron (III) ions (Fe3+) or bivalent metal ions other than iron. It also does not react to chelated iron in ferritin and other substances. FerroOrange is suitable for live-cell imaging because it is highly cell-permeable and has low cell toxicity.Spectral Properties:Absorbance (max): 542 nmEmission (max): 572 nmResearch Category:Live Cell Dye

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

질문

1–4 / 4 질문  

  1. Can this kit be used for suspension cells?

    1 답변

    1. Yes, this product is suitable for use with suspension cells.

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  2. Is it possible to store SCT210 after reconstitution?

    1 답변

    1. The available datasheet for SCT210 provides guidance in the Reagent Preparation section, stating, "Use neutral buffer or culture medium for dilution of the DMSO solution and use immediately upon dilution. The dye could be oxidized in acidic solutions." However, there are no recommendations or data suggesting that the solutions are stable when stored. It's important to note that the package is shipped in single-use vials.

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  3. Is it possible to detect SCT210 FerroOrange by flow cytometry?

    1 답변

    1. The website indicates an excitation wavelength of 542 nm and an emission wavelength of 572 nm for the SCT210 FerroOrange. The product is claimed to be suitable for live cell imaging, which typically implies the use of a confocal microscope. However, given that this is a fluorescent dye, it seems reasonable to consider that the staining could be assessed using a flow cytometer. There is currently no available data documenting its use on a flow cytometer. To use it on a flow cytometer, the cells would need to be in a single cell suspension. Additionally, it is expected that the cells could be visualized using a fluorescent microscope.

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  4. What cells can be used as a positive control for SCT210 (BioTracker™ FerroOrange Live Cell Dye)?

    1 답변

    1. The HepG2 cell line is recommended as a positive control for SCT210 (BioTracker™ FerroOrange Live Cell Dye).

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