MABN757

Anti-Beta-glucosidase 2 (GBA2) Antibody, clone 4A12

clone 4A12, from rat

• 동의어(들): Non-lysosomal glucosylceramidase, Beta-glucocerebrosidase 2, Beta-glucosidase 2, Glucosylceramidase 2, NLGase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 4A12, monoclonal
• 종 반응성: mouse
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_766280
• UniProt 수납 번호: Q69ZF3
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Gba2(230101)

설명

일반 설명

Non-lysosomal glucosylceramidase (EC 3.2.1.45; UniProt Q69ZF3; also known as Beta-glucocerebrosidase 2, Beta-glucosidase 2, Glucosylceramidase 2, NLGase) is encoded by the Gba2 (also known as Kiaa1605) gene (Gene ID 230101) in murine species. Glucosidases GBA1, GBA2, and GBA3 are structurally unrelated proteins with similar hydrolase activity that mediates glucosylceramide (GlcCer) degradation. GBA1 mediates GlcCer degradation in the lysosome lumen following its transport via the endosomal pathway. GBA2 is localized at the cytosolic surface of the ER and Golgi, with a predominant presence at the cis-Golgi in close proximity to the site of GlcCer synthesis and sphingomyelin conversion. GlcCer is also delivered to the ER through FAPP2-dependent transport and then flips to ER lumenal side by low specificity phospholipid flippases. GBA2 ER localization allows it to regulate GlcCer level at the cytosolic side of the ER and limit the amount of GlcCer from entering the ER lumenal side for higher order glycosphingolipid synthesis. Impaired GBA1 activity causes accumulation of GlcCer in lysosomes of tissue macrophages, causing liver and spleen enlargement and impairment of the central nervous system in patients with Gaucher disease. GBA2 activity is found downregulated in fibroblasts from a patient with type II Gaucher disease and fibroblasts from Gba1-deficient mice, suggesting a cross-talk between GBA1 and GBA2 that could affect the phenotype of Gaucher disease. GBA3 is a cytosolic Klotho-related protein whose physiological function is not yet known.

특이성

Clone 4A12 detected the beta-glucosidase 2 (GBA2) target band in testis tissue sample from wild-type, but not Gba2-knock mice (Körschen, H.G., et al. (2013). J. Biol. Chem. 288(5):3381-3393).

면역원

Ovalbumin-conjugated linear peptide corresponding to a sequence from the N-terminal region of mouse beta-glucosidase 2 (GBA2).

애플리케이션

Anti-Beta-glucosidase 2 (GBA2) Antibody, clone 4A12, Cat. No. MABN757, is a highly specific rat monoclonal antibody that targets beta-glucosidase 2 (GBA2) and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized, but not non-permeabilized, HEK293 transfectants expressing HA-tagged mouse beta-glucosidase 2 (GBA2-HA). GBA2-HA immunoreactivity was found associated with both Golgi and ER markers and greatly dependent on microtubule dynamics (Körschen, H.G., et al. (2013). J. Biol. Chem. 288(5):3381-3393).

Immunocytochemistry Analysis: A representative lot detected beta-glucosidase 2 (GBA2) in isolated embryonic mouse hippocampal neurons, but not astrocytes (Körschen, H.G., et al. (2013). J. Biol. Chem. 288(5):3381-3393).

Western Blotting Analysis: A representative lot detected HA-tagged mouse beta-glucosidase 2 exogenously expressed in HEK293 cells, as well endogenous beta-glucosidase 2 in testis tissue samples from wild-type, but not Gba2-knock mice (Körschen, H.G., et al. (2013). J. Biol. Chem. 288(5):3381-3393).

Research Category
Neuroscience

품질

Evaluated by Immunohistochemistry in mouse brain tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected beta-glucosidase 2 (GBA2) in mouse brain tissue sections.

표적 설명

103.3 kDa calculated.

물리적 형태

Format: Purified

Protein G purified.

Purified rat IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable