MABN1844

Anti-Aldolase C Antibody, C-Term, clone 1A1

clone 1A1, from mouse

• 동의어(들): Fructose-bisphosphate aldolase C, Brain-type aldolase, Aldolase C, C-Term
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 1A1, monoclonal
• 종 반응성: human, rat
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_005156
• UniProt 수납 번호: P09972
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ALDOC(230)

설명

일반 설명

Fructose-bisphosphate aldolase C (EC 4.1.2.13; UniProt P09972; also known as Aldolase 3, Brain-type aldolasefructoaldolase C, Fructose-1,6-biphosphate triosephosphate lyase) is encoded by the ALDOC (also known as ALDC) gene (Gene ID 230) in human. Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Three aldolase genes (ALDOA, ALDOB, ALDOC) are found in mammals that encode aldolase A, B, and C. Aldolase A is generally considered to be a muscle enzyme, aldolase B a liver enzyme, while aldolase C shows tissue-specific expression in adult brain with low activity in fetal tissues. Aldolase C expression is found in gray matter astrocytes and cells of the pia mater. Aldolase A and C mRNA expressions are mutually exclusive in murine central nervous system by in situ hybridization analysis, with aldolase A mRNA being detected in neurons and aldolase C in astrocytes, Purkinje cells, and some cells of the pia mater. Aldolase C can therefore be a useful marker of astrocytes, although Purkinje cells of the cerebellum also contain high levels of aldolase C.

면역원

Epitope: Near C-terminus; 23 amino acids of aldolase C protein

Recombinant protein corresponding to human Aldolase C near the C-terminus.

애플리케이션

This Anti-Aldolase C Antibody, C-Term, clone 1A1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Aldolase C.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Aldolase C in 10 µg of human brain tissue lysate.
Immunocytochemistry: A representative lot selectively stained Aldolase C-positive astrocytes in mixed neuron/glial cultures.

품질

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Aldolase C in 10 µg of rat brain tissue lysate.

표적 설명

~39 kDa observed

물리적 형태

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing PBS with 0.05% sodium azide with 50% glycerol.

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable