MABF3091

Anti-LMP1 Antibody, clone 1G6

• 동의어(들): LMP-1, Protein p25, Protein p63
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rat
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 1G6, monoclonal
• 분자량: calculated mol wt 42 kDa; observed mol wt ~N/A kDa
• 정제법: using protein G
• 종 반응성: virus
• 포장: antibody small pack of 100
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG2a
• 에피토프 서열: C-terminal cytoplasmic domain
• 단백질 ID 수납 번호: N/A
• UniProt 수납 번호: P03230
• 저장 온도: 2-8°C
• 유전자 정보: vaccinia virus ... LMP1(3783750)

설명

특이성

Clone 1G6 is a rat monoclonal antibody that detects LMP1. It targets an epitope within the C-terminal cytoplasmic domain of the protein.

면역원

Wild type LMP1-TrpE-fusion protein from Epstein-Barr virus.

애플리케이션

Quality Control Testing

Evaluated by Immunocytochemistry in B95-8 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected LMP1 in B95-8 cells.

Tested Applications

Immunohistochemistry Analysis: A representative lot detected LMP1 in Immunohistochemistry applications (Nicholls, J., et al. (2004). J Virol Methods. 116(1):79-88; Shair, K.H.Y., et al. (2007). PLoS Pathog. 3(11):e166).

Immunocytochemistry Analysis: A representative lot detected LMP1 in Immunocytochemistry applications (Nicholls, J., et al. (2004). J Virol Methods. 116(1):79-88).

Western Blotting Analysis: A representative lot detected LMP1 in Western Blotting applications (Nicholls, J., et al. (2004). J Virol Methods. 116(1):79-88; Thornburg, N.J., et al. (2006). Oncogene. 25(2):288-97; Hannigan, A., et al. (2010). Mol Cancer. 9:9:184; Rancan, C., et al. (2015). PLoS Pathog. 11(6):e1004906).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Latent membrane protein 1 (LMP1) (UniProt: P03230) is encoded by the LMP1 (also known as BNLF1) gene (Gene ID: 3783750) in Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4). Latent membrane protein 1 (LMP1) is a viral oncoprotein encoded by the Epstein-Barr virus (EBV) and is known for its critical role in EBV-associated oncogenesis. LMP1 functions as a constitutively active mimic of the tumor necrosis factor receptor (TNFR) and can activate various signaling pathways, including NF- B, JNK, and PI3K/Akt. Through its ability to modulate these pathways, LMP1 can promote cell proliferation, inhibit apoptosis, and induce the expression of various cytokines and growth factors. Additionally, LMP1 contributes to the immortalization of infected B cells and is implicated in the pathogenesis of EBV-associated malignancies, including Hodgkin′s lymphoma and nasopharyngeal carcinoma.

물리적 형태

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable