MABF264

Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody

clone 13F10, from rat

• 동의어(들): WD repeat-containing protein 1, Actin-interacting protein 1, AIP1, AIP1/WD repeat-containing protein 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 300
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 13F10, monoclonal
• 종 반응성: mouse
• 기술: western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_035845
• UniProt 수납 번호: O88342
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Wdr1(22388)

설명

일반 설명

Actin-interacting protein 1 (AIP1), also known as WD repeat-containing protein 1 (Wdr1), is a key player in cytoskeletal rearrangements and actin mobilization, and thus plays a role in many cellular situations from movement to cytokinesis. AIP1 interacts with Cofilin as well, and Cofilin regulates actin filament dynamics by stimulating actin filament disassembly and also plays a critical role in cytokinesis and chemotactic migration. Knockdown of AIP1 in HeLa cells increased the percentage of multinucleate cells and the mechanics of cytokinesis were altered with an excessive buildup of actin filaments at telophase. The results could be relieved by overexpression of Cofilin pointing to AIP1’s critical role in Cofilin-mediated actin filament disassembly. Furthermore, AIP1 inhibition caused abnormal cell migration in response to cytokine stimulation leading to a non-directional migration phenotype thus indicating that AIP1 plays an important directional role in response to stimuli as well. Finally AIP1, as one might expect, plays a critical role in development of cells and tissues, especially in epithelial morphogenesis for instance.

면역원

Recombinant protein corresponding to mouse AIP1/WD repeat-containing protein 1.

애플리케이션

This Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody is validated for use in Western Blotting for the detection of AIP1/WD repeat-containing protein 1.

Western Blotting Analysis: A representative lot detected AIP1/WD repeat-containing protein 1 in both endogenous and transfected mouse AIP1 in western blots of cell lysates from J774 cells, 293T cells and 293T cells transfected with HA-tagged mouse Aip1 (Li, J., et al. (2007). Nature. 9(3):276-286).

품질

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected AIP1/WD repeat-containing protein 1 in 10 µg of RAW264.7 cell lysate.

표적 설명

~66 kDa observed

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 10 - Combustible liquids