MABE224

Anti-ZFX Antibody, clone 3E8.1

clone 3E8.1, from mouse

• 동의어(들): Zinc finger X-chromosomal protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 3E8.1, monoclonal
• 종 반응성: human
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_001171555
• UniProt 수납 번호: P17010
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ZFX(7543)

설명

일반 설명

ZFX, also known as Zinc finger X-chromosomal protein, and encoded by the gene name ZFX, is probable transcriptional activator. This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined.

면역원

GST-tagged recombinant protein corresponding to human ZFX.

애플리케이션

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected ZFX HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-ZFX antibody is validated for use in WB, IC for the detection of ZFX.

품질

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZFX in 200 µg of HeLa nuclear extract.

표적 설명

~130, ~90, ~59 kDa observed. Three bands (~130 kDa, ~91 kDa and ~59 kDa) corresponding to ZFX were detected. The ~130 kDa band is nuclear, while the ~91 kDa band was found in the cytoplasmic (Zhu, Z., et al. (2013). J Neurooncol. 112:17-25).

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2