MABE1121

Anti-Ago1 Antibody, clone 4B8

clone 4B8, from rat

• 동의어(들): Protein argonaute-1, Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding protein Q99, Ago1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 4B8, monoclonal
• 종 반응성: human
• 기술: western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_036331.1
• UniProt 수납 번호: Q9UL18
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... AGO1(26523)

설명

일반 설명

Protein argonaute-1 (UniProt Q9UL18; also known as Argonaute1, Argonaute RISC catalytic component 1, eIF-2C 1, eIF2C 1, Eukaryotic translation initiation factor 2C 1, hAgo1, Putative RNA-binding protein Q99) is encoded by the AGO1 (also known as EIF2C, EIF2C1) gene (Gene ID 26523) in human. Ago1 belongs to the family of argonaute proteins involved in gene expression by regulating gene transcription and RNA splicing in the nucleus, as well as by regulating posttranscriptional gene silencing mediated by siRNAs and microRNAs in the cytosol. Ago1 is shown to interact with chromatin modifiers, splicing factors, and RNA polymerase II. Ago1 and the related Ago2 are also shown to target Hepatitis C Virus (HCV) RNÁ 5′-untranslated region (5′-UTR) in an miR-122-dependent manner, resulting in viral RNA stability and translation stimulation.

특이성

Clone 4B8 sepcifically immunoprecipitated FLAG-tagged Ago1,but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528).

면역원

GST-tagged recombinant human Ago1.

애플리케이션

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Ago1 in 10 µg of K562 cell lysate.
Western Blotting Analysis: A representative lot detected Ago1-containing messenager ribonucleoprotein (mRNP) complexes primarily in the low density fractions of sucrose gradient-fractionated HEK293 lysate (Höck, J., et al. (2007). EMBO Rep. 8(11):1052-1060).
Immunoprecipitation Analysis: Representative lots immunoprecipitated FLAG-tagged Ago1, but not FLAG-tagged Ago2, Ago3, or Ago4 (Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).
RNA Binding Protein Immunoprecipitation (RIP): Representative lots co-immunoprecipitated Ago1-associated RNAs, including mRNAs, miRNAs, and snoRNAs from human cell lysates (Li, Z., et al. (2009). J. Virol. 83(24):12751-12758; Ender, C., et al. (2008). Mol. Cell. 32(4):519-528; Beitzinger, M., et al. (2007). RNA Biol. 4(2):76-84).

품질

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ago1 in 10 µg of HeLa cell lysate.

표적 설명

~97 kDa observed

물리적 형태

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable