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생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
2A2, monoclonal
종 반응성
mouse, human
기술
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1κ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
human ... ING3(54556)
일반 설명
Inhibitor of growth protein 3 (UniProt: Q9NXR8; also known as ING3, p47ING3) is encoded by the ING3 (also known as HSPC301) gene (Gene ID 54556) in human. ING3 is a ~50 kDa protein that belongs to a family of proteins that contain PHD-finger domain and act as readers of the epigenetic code through specific recognition of trimethylated lysine 4 of histone H3. ING3 is a component of the NuA4 histone acetyltransferase (HAT) complex, which is involved in transcriptional activation of select genes, principally by acetylation of nucleosomal histones H4 and H2A. This complex is also shown to activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. ING3 is highly expressed in small intestine, bone marrow, epidermis, and in tissues where cells undergo rapid proliferation and renewal and its expression is shown to be higher in proliferating cells. Overexpression of ING3 has been shown to inhibit cell growth and induce apoptosis. ING3 is reported to be down-regulated in squamous cell carcinoma of the head and neck.
특이성
Clone 2A2 detected recombinant human ING3, but not INGs 1, 2, 4 or 5. Target band detection by Western blotting and immunocytochemistry. Staining by clone 2A2 was abolished using ING3 shRNA-transfected cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
면역원
GST-tagged recombinant full-length human ING3.
애플리케이션
Detect ING3 using this mouse monoclonal Anti-ING3 Antibody, clone 2A2, Cat. No. MABC1185, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ING3 immunoreactivity in human small intestine tissue section.
Immunocytochemistry Analysis: A representative lot detected upregulated and downregulated ING3 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells transfected with ING3 and ING3 shRNA, respectively. Preabsorption with fixed ING3-overexpressing cells prevented subsequence immunocytochemical staining by clone 2A2 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunohistochemistry Analysis: A representative lot detected differential ING3 immunoreactivity among formalin-fixed paraffin-embedded human tissue sections, inluding hematopoietic tissues (bone marrow, lymphatic, thymus, and spleen) and epithelium of various tissue types (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunoprecipitation Analysis: A representative lot immunoprecipitated HA-tagged ING3 exogenously expressed in HEK293 cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Western Blotting Analysis: A representative lot detected endogenous ING3 in human HEK293 cells, a panel of murine tissues and cells (BALB-3T3 and NIH-3T3) as well as exogenously overexpressed ING3 in HEK293 cells, ING3 shRNA transfection abolished target band detection. Clone 2A2 detected bacterially rexpressed recombinant human ING3, but not INGs 1, 2, 4 or 5 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Western Blotting Analysis: A representative lot detected growth factor-induced ING3 expression in serum-starved HME human mammary fibroblasts immortalized with hTERT and RWPE-1 prostate epithelial cells immortalized with HPV18 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunocytochemistry Analysis: A representative lot detected upregulated and downregulated ING3 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells transfected with ING3 and ING3 shRNA, respectively. Preabsorption with fixed ING3-overexpressing cells prevented subsequence immunocytochemical staining by clone 2A2 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunohistochemistry Analysis: A representative lot detected differential ING3 immunoreactivity among formalin-fixed paraffin-embedded human tissue sections, inluding hematopoietic tissues (bone marrow, lymphatic, thymus, and spleen) and epithelium of various tissue types (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Immunoprecipitation Analysis: A representative lot immunoprecipitated HA-tagged ING3 exogenously expressed in HEK293 cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Western Blotting Analysis: A representative lot detected endogenous ING3 in human HEK293 cells, a panel of murine tissues and cells (BALB-3T3 and NIH-3T3) as well as exogenously overexpressed ING3 in HEK293 cells, ING3 shRNA transfection abolished target band detection. Clone 2A2 detected bacterially rexpressed recombinant human ING3, but not INGs 1, 2, 4 or 5 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
Western Blotting Analysis: A representative lot detected growth factor-induced ING3 expression in serum-starved HME human mammary fibroblasts immortalized with hTERT and RWPE-1 prostate epithelial cells immortalized with HPV18 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
품질
Evaluated by Immunohistochemistry in human ovarian cancer tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ING3 immunoreactivity in human ovarian cancer tissue section.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ING3 immunoreactivity in human ovarian cancer tissue section.
표적 설명
46.74 kDa calculated. ~50 kDa reported (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).
물리적 형태
Format: Purified
기타 정보
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Alessandra Chesi et al.
Nature communications, 10(1), 1260-1260 (2019-03-21)
Osteoporosis is a devastating disease with an essential genetic component. GWAS have discovered genetic signals robustly associated with bone mineral density (BMD), but not the precise localization of effector genes. Here, we carry out physical and direct variant to gene
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